Related Experiment Video
Updated: Apr 15, 2026

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
Published on: December 27, 2016
Compartmentalization in penicillin G biosynthesis by Penicillium chrysogenum PQ-96
Abstract:
The arrangement of organelles in the sub-apical productive non-growing vacuolated hyphal cells of the high- and the low-penicillin-pro- ducing strains Penicillium chrysogenum was compared using transmission electron microscopy. In the productive cells of the high-yielding strain the endoplasmic reticulum and the polyribosomes with associated peroxisomes are frequently arranged at the periphery of the cytoplasm and around the vacuoles. At the high activity of penicillin G biosynthesis the immuno-label of the cytosolic isopenicillin N synthase is concentrated at the polyribosomes arranged in the peripheral cytoplasm and along the tonoplast as well as around the peroxisomes. On the basis of the obtained results the compartmentalization of the pathway of penicillin G biosymthesis is discussed. The obtained results support the phenylacetic acid detoxification hypothesis of penicillin G biosynthesis.
Related Concept Videos
Production of Antibiotics
Inhibitors of Gram-positive Cell Wall Synthesis
Production of Pharmaceuticals
Peptidoglycan Synthesis
Gene Regulation in Microbial Communities: Quorum Sensing
Biosynthesis in Bacteria

