Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofuels01:25

Biofuels

96
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
96
Green Algae01:21

Green Algae

1.1K
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
1.1K
Lipid Catabolism01:25

Lipid Catabolism

1.4K
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1.4K
Microbial Bioremediation of Hydrocarbons01:26

Microbial Bioremediation of Hydrocarbons

97
Bioremediation is an environmentally sustainable process that employs living organisms—primarily microorganisms—to degrade or neutralize pollutants from contaminated environments. In oil spills and hydrocarbon pollution, bioremediation involves the use of hydrocarbon-degrading bacteria to transform toxic compounds into less harmful substances. This approach leverages natural microbial metabolic processes and is considered both cost-effective and ecologically favorable compared to...
97
Microbial Leaching01:27

Microbial Leaching

135
Microbial leaching, also known as bioleaching, is an environmentally favorable method for extracting metals from low-grade ores using specific microorganisms. This biotechnological approach is particularly valuable for mining operations targeting copper, gold, and uranium, where traditional extraction methods may be economically or environmentally impractical.Copper Leaching and Microbial CatalysisIn copper bioleaching, crushed ore is arranged into heaps and irrigated with a dilute sulfuric...
135

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic Reconstruction of Cu Catalyst Under Electrochemical NO Reduction to NH<sub>3</sub>.

ChemSusChem·2024
Same author

Enhanced astaxanthin production in Haematococcus lacustris by electrochemical stimulation of cyst germination.

Bioresource technology·2024
Same author

Optimizing electroactive membrane performance for microalgae harvesting: A response surface methodology study of membrane formulation and operating parameters for electro filtration.

Chemosphere·2023
Same author

Development of dual strain microalgae cultivation system for the direct carbon dioxide utilization of power plant flue gas.

Bioresource technology·2023
Same author

Electrochemically-mediated reactive separation of nitric oxide into nitrate using iron chelate.

Chemosphere·2023
Same author

Simultaneous enhancement of lipid biosynthesis and solvent extraction of Chlorella using aminoclay nanoparticles.

Bioresource technology·2023

Related Experiment Video

Updated: Apr 15, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.5K

Lipid extraction and esterification for microalgae-based biodiesel production using pyrite (FeS2).

Yeong Hwan Seo1, Mina Sung1, You-Kwan Oh2

  • 1Department of Civil and Environmental Engineering, KAIST, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.

Bioresource Technology
|March 26, 2015
PubMed
Summary

Pyrite (FeS2) effectively extracts lipids and aids esterification for microalgae biodiesel. Oxidized pyrite matches commercial catalyst efficiency, offering a cheaper, sustainable alternative for biofuel production.

Keywords:
Fenton-like reactionFerric chlorideLipid extractionPyriteTwo-step esterification

More Related Videos

Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

61.8K
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

22.9K

Related Experiment Videos

Last Updated: Apr 15, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

21.5K
Analysis of Fatty Acid Content and Composition in Microalgae
07:44

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

61.8K
Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids
11:08

Cultivation of Green Microalgae in Bubble Column Photobioreactors and an Assay for Neutral Lipids

Published on: January 7, 2019

22.9K

Area of Science:

  • Biomass and Biofuel Production
  • Green Chemistry
  • Catalysis

Background:

  • Microalgae are a sustainable source for biodiesel.
  • Efficient lipid extraction and esterification are crucial for biodiesel production.
  • Current catalysts can be expensive or lead to undesirable side reactions like saponification.

Purpose of the Study:

  • To investigate pyrite (FeS2) as a low-cost catalyst for microalgae lipid extraction.
  • To evaluate oxidized pyrite for esterification of free fatty acids into fatty acid methyl esters (biodiesel).
  • To assess the feasibility of using pyrite as a dual-purpose catalyst in biodiesel production.

Main Methods:

  • Lipid extraction using pyrite via an iron-mediated oxidation (Fenton-like) reaction.
  • Comparison of pyrite's efficiency with commercial ferric chloride (FeCl3).
  • Esterification of free fatty acids using oxidized pyrite under acidic conditions.

Main Results:

  • Pyrite showed moderate lipid extraction efficiency, improved significantly upon oxidation.
  • Oxidized pyrite achieved approximately 90% lipid extraction efficiency, comparable to FeCl3 at 20 mM.
  • Oxidized pyrite facilitated esterification with over 90% efficiency, avoiding saponification.

Conclusions:

  • Pyrite is a viable, cost-effective catalyst for both lipid extraction and esterification in microalgae biodiesel production.
  • Oxidized pyrite offers comparable efficiency to commercial catalysts, presenting a sustainable alternative.
  • Utilizing pyrite circumvents saponification issues associated with alkaline catalysts.