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Influenza Virus Propagation in Embryonated Chicken Eggs
Published on: March 19, 2015
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Isolation of chicken astrovirus from specific pathogen-free chicken embryonated eggs
Luis Fabian N Nuñez1, Silvana H Santander Parra1, Elena Mettifogo1
1Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando M. Paiva, 87, 05508-270, São Paulo, SP, Brazil.
Poultry Science
|March 26, 2015
Summary
Chicken astrovirus (CAstV) was isolated and characterized in specific pathogen-free chicken embryonated eggs (CEE). This study details the pathological effects of CAstV in CEE, providing insights into avian enteric disease.
Area of Science:
- Veterinary Virology
- Avian Pathology
- Molecular Diagnostics
Background:
- Chicken astroviruses (CAstV) are implicated in enteric diseases in poultry.
- Runting-stunting syndrome and diarrhea are significant concerns in chicken flocks.
- Understanding CAstV pathogenesis is crucial for disease control.
Purpose of the Study:
- To isolate and propagate chicken astrovirus (CAstV) in specific pathogen-free chicken embryonated eggs (CEE).
- To characterize the pathological features of CAstV infection in CEE.
- To differentiate CAstV from other potential avian enteric pathogens.
Main Methods:
- Isolation of CAstV from diarrheic chickens with runting-stunting syndrome.
- Inoculation of CEE via the yolk sac route.
- Viral confirmation using PCR and transmission electron microscopy.
- Differential diagnostic screening for common avian enteric viruses.
Main Results:
- Successful isolation and propagation of CAstV in CEE.
- Pathological findings in 7- or 14-day-old CEE included hemorrhages, edema, gelatinous appearance, deformities, and dwarfism.
- Exclusion of co-infection with avian nephritis virus, rotavirus, reovirus, parvovirus, infectious bronchitis virus, and fowl adenovirus Group I.
Conclusions:
- Chicken astrovirus (CAstV) can be effectively isolated and propagated in specific pathogen-free chicken embryonated eggs (CEE).
- CAstV induces characteristic pathological changes in CEE, including developmental abnormalities.
- This model provides a valuable tool for studying CAstV and associated avian enteric diseases.
