Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translational Regulation01:29

Translational Regulation

875
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
875
Global Regulatory Systems01:28

Global Regulatory Systems

927
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
927
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

1.1K
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1.1K
Types of RNA01:23

Types of RNA

74.2K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
74.2K
Types of RNA01:20

Types of RNA

16.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.7K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

12.3K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.

Scientific reports·2026
Same author

Genome-guided antimicrobial potential of Bacillus stercoris from coastal sand with activity against multidrug-resistant bacteria, including MRSA.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Regulatory RNA Ern0160 controls Enterococcus faecium virulence through direct modulation of expression of LysM domain-containing proteins.

BMC genomics·2026
Same author

sRNA-mediated crosstalk between cell wall stress and galactose metabolism in Staphylococcus aureus.

Nucleic acids research·2025
Same author

Exploring the interactome of the Staphylococcus aureus sRNA Srn_9342 identified a complex formation with RNAIII leading to the modulation of δ-hemolysin expression.

BMC microbiology·2025
Same author

<i>Lactobacillus delbrueckii</i> subsp. <i>allosunkii</i> and <i>lactis</i> as emerging human uropathogens in elderly patients.

Journal of clinical microbiology·2025
Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026
Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026
Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026
Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026
Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026
Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Apr 15, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.7K

SRD: a Staphylococcus regulatory RNA database.

Mohamed Sassi1, Yoann Augagneur1, Tony Mauro1

  • 1Inserm U835 Biochimie Pharmaceutique, Rennes University, 35043 Rennes, France.

RNA (New York, N.Y.)
|March 26, 2015
PubMed
Summary
This summary is machine-generated.

A new database, SRD, catalogs bacterial regulatory RNAs (sRNAs), focusing on Staphylococcus aureus. It provides curated sequences, experimental evidence, and prediction tools for these small RNAs.

Keywords:
RNA-seqStaphylococcus aureusbacteriadatabasesRNA identificationsRNA targetssmall regulatory RNAs

More Related Videos

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.6K
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.2K

Related Experiment Videos

Last Updated: Apr 15, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.7K
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

9.6K
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.2K

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Bacterial small regulatory RNAs (sRNAs) are crucial for gene expression.
  • Hundreds of sRNAs have been identified across bacterial species, including pathogens like Staphylococcus aureus.
  • A centralized resource for Staphylococcal sRNAs was lacking.

Purpose of the Study:

  • To create a comprehensive database of Staphylococcal regulatory RNAs (sRNAs).
  • To provide curated data, novel identifiers, and analysis tools for sRNAs.
  • To facilitate research on sRNA function and regulation in Staphylococci.

Main Methods:

  • Compilation of published sRNA data for Staphylococci.
  • Development of the Staphylococcal Regulatory RNA Database (SRD).
  • RNA-sequencing analysis for annotation and identification of novel sRNAs.
  • Genome-wide prediction and comparative analysis of sRNAs across Staphylococcal species.
  • Integration of prediction tools (MFold, intaRNA) and BLAST server.

Main Results:

  • SRD provides a curated list of 575 unique Staphylococcal regulatory RNAs (srn).
  • RNA-seq analysis identified 159 additional independent transcribed sRNAs.
  • Comparative genomics revealed poor sRNA conservation across Staphylococcal species.
  • The database includes genetic locations, sequences, experimental evidence, and structure predictions.

Conclusions:

  • SRD is the first genus-specific database for bacterial regulatory RNAs.
  • The database offers a user-friendly platform for accessing and analyzing Staphylococcal sRNAs.
  • SRD will aid in understanding sRNA roles in bacterial physiology and pathogenesis.