Related Experiment Video
Updated: Apr 15, 2026
Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease
Published on: May 10, 2024
Protective effect of some selected fruit blends on testicular toxicity in alloxan-induced diabetic rats
Background:
The protective effect of a developed drink from blends of selected fruits on the sperm quality of alloxan-induced diabetic rats was investigated.
Methods:
Diabetes was intraperitoneally induced with alloxan; the formulated drink blend was orally administered 2.5 or 5 mL/day. Treatment lasted for 14 days and the rats were humanely sacrificed by cervical dislocation. The antioxidant status via assessment of reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and lipid peroxidation (LPO) was carried out on the testis, while sperm cells were analyzed for sperm motility, counts and abnormality.
Results:
Induction of diabetes led to a significant (p < 0.05) decrease in GSH level, elevated SOD and CAT activities, significant (p < 0.05) decrease in the sperm quality parameters studied. However, treatment with the formulated drink led to a significant (p < 0.05) reduction of LPO, SOD and CAT activities as well as increase in GSH level.
Conclusions:
This study shows an improvement in testicular antioxidant activities and sperm qualities by single and double doses of the formulation, suggesting its protective potential against testicular toxicity in diabetic rats.
Insights
This study demonstrates that a fruit blend drink improves sperm quality and testicular antioxidant status in diabetic rats, offering protection against diabetes-induced toxicity.
Area of Science:
- Reproductive Biology
- Toxicology
- Nutritional Science
Background:
- Diabetes mellitus is associated with impaired male reproductive function.
- Alloxan-induced diabetes in rats serves as a model to study diabetes-related testicular toxicity.
- Oxidative stress plays a crucial role in diabetic complications affecting sperm quality.
Purpose of the Study:
- To investigate the protective effects of a fruit blend drink on sperm quality in alloxan-induced diabetic rats.
- To evaluate the impact of the fruit drink on testicular antioxidant status.
- To determine the efficacy of different doses of the fruit drink formulation.
Main Methods:
- Diabetes was induced using intraperitoneal alloxan injection.
- Rats were treated with the fruit blend drink at doses of 2.5 or 5 mL/day for 14 days.
- Testicular antioxidant markers (reduced glutathione, catalase, superoxide dismutase, lipid peroxidation) and sperm parameters (motility, count, abnormality) were assessed.
Main Results:
- Alloxan-induced diabetes significantly reduced sperm quality and altered testicular antioxidant status (decreased GSH, increased SOD and CAT, elevated LPO).
- Treatment with the fruit blend drink significantly improved sperm parameters.
- The drink formulation effectively reduced lipid peroxidation and normalized superoxide dismutase and catalase activities, while increasing reduced glutathione levels.
Conclusions:
- The developed fruit blend drink demonstrates a protective effect against testicular toxicity in diabetic rats.
- Administration of the fruit drink improved testicular antioxidant activities and sperm quality parameters.
- The findings suggest the potential therapeutic value of this fruit formulation in managing diabetes-related reproductive dysfunction.
