Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic
Malgorzata Broncel1, Remigiusz A Serwa, Paulina Ciepla
1Department of Chemistry, Imperial College London, Exhibition Road, London SW7 2AZ (UK).
Abstract:
Novel multifunctional reagents were applied in combination with a lipid probe for affinity enrichment of myristoylated proteins and direct detection of lipid-modified tryptic peptides by mass spectrometry. This method enables high-confidence identification of the myristoylated proteome on an unprecedented scale in cell culture, and allowed the first quantitative analysis of dynamic changes in protein lipidation during vertebrate embryonic development.
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