Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent morphological probe for hyaluronate.

C B Knudson, B P Toole

    The Journal of Cell Biology
    |May 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a novel fluorescent probe to visualize hyaluronate, a molecule crucial for tissue development. This probe allows for the study of hyaluronate distribution, aiding in understanding its role in morphogenesis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts.

    BMC cancer·2019
    Same author

    Extracellular matrix recovery by human articular chondrocytes after treatment with hyaluronan hexasaccharides or Streptomyces hyaluronidase.

    Modern rheumatology·2014
    Same author

    High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase.

    Osteoarthritis and cartilage·2013
    Same author

    Cooperative roles for emmprin and LYVE-1 in the regulation of chemoresistance for primary effusion lymphoma.

    Leukemia·2011
    Same author

    Hyaluronan oligosaccharide treatment of chondrocytes stimulates expression of both HAS-2 and MMP-3, but by different signaling pathways.

    Osteoarthritis and cartilage·2009
    Same author

    Hyaluronan promotes the chondrocyte response to BMP-7.

    Osteoarthritis and cartilage·2009
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Developmental Biology

    Background:

    • Hyaluronate is vital for tissue and organ morphogenesis.
    • Understanding hyaluronate's spatial and temporal distribution is key to elucidating its role in development.
    • Current methods lack direct visualization of hyaluronate using microscopy.

    Purpose of the Study:

    • To develop a novel method for visualizing hyaluronate distribution.
    • To create a specific and sensitive probe for hyaluronate detection.
    • To investigate cell surface hyaluronate in different cell types.

    Main Methods:

    • Developed a fluorescent probe using cartilage proteoglycan core protein.
    • Modified the core protein by removing sulfated polysaccharide chains.

    Related Experiment Videos

  • Labeled the core protein with rhodamine for fluorescence detection.
  • Validated probe specificity and binding affinity for hyaluronate using binding assays.

    • Main Results:

    • The rhodamine-labeled proteoglycan core protein specifically binds to hyaluronate.
    • The probe demonstrated reversible binding to hyaluronate.
    • Successfully visualized cell surface hyaluronate on rat fibrosarcoma, 3T3, and SV-40 transformed 3T3 cells.
    • Observed significant differences in cell surface hyaluronate levels among the tested cell types.

    Conclusions:

    • A novel fluorescent probe enables specific visualization of hyaluronate.
    • This probe facilitates the study of hyaluronate's role in morphogenesis.
    • The method allows for quantitative and qualitative analysis of cell surface hyaluronate.