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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Genetic variability of blueberry scorch virus isolates from highbush blueberry in New York State
Elżbieta Kalinowska1, Patricia Marsella-Herrick, Marc Fuchs
1Department of Plath Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02-776, Warsaw, Poland, ela_kalinowska@op.pl.
Abstract:
The genetic variability of blueberry scorch virus (BlScV) isolates from New York was determined within a portion of the RNA-dependent RNA polymerase gene and the triple gene block and coat protein (CP) genes. Phylogenetic analysis of 19 New York isolates and other isolates for which sequence information is available in GenBank revealed two distinct clades, regardless of the coding region analyzed, and limited variability within (0.029 ± 0.007) and between (0.183 ± 0.032) phylogroups. Recombination events were identified in the CP gene of three New York isolates, and codons of the five BlScV genes characterized were found to be under neutral or negative selective pressure.
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