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Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
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S-sulfhydration: a cysteine posttranslational modification in plant systems
Ángeles Aroca1, Antonio Serna1, Cecilia Gotor1
1Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas y Universidad de Sevilla, 41092 Seville, Spain (A.A., C.G., L.C.R.); andAB Sciex, 28108 Alcobendas, Madrid, Spain (A.S.).
Plant Physiology
|March 27, 2015
Summary
Hydrogen sulfide (H2S) acts as a signaling molecule in plants. This study identified 106 S-sulfhydrated proteins in Arabidopsis, revealing H2S reversibly regulates plant protein function.
Area of Science:
- Biochemistry
- Plant Physiology
- Molecular Biology
Background:
- Hydrogen sulfide (H2S) is recognized as a crucial signaling molecule in mammals, plants, and other organisms.
- While H2S physiological roles are known, the precise molecular mechanisms remain largely unelucidated.
- Post-translational modification of proteins, specifically S-sulfhydration of cysteine residues, is a proposed mechanism for H2S action.
Purpose of the Study:
- To detect and identify S-sulfhydrated proteins in Arabidopsis thaliana under physiological conditions.
- To investigate the role of S-sulfhydration in regulating plant protein function.
Main Methods:
- Utilized a modified biotin switch assay for detecting S-sulfhydration.
- Employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) for protein identification.
- Conducted immunoblot and enzyme activity assays to validate functional regulation.
Main Results:
- Successfully identified 106 S-sulfhydrated proteins in Arabidopsis.
- Confirmed S-sulfhydration of cytosolic ascorbate peroxidase.
- Demonstrated that S-sulfhydration reversibly modulates the activity of plant proteins.
Conclusions:
- S-sulfhydration is a significant mechanism for hydrogen sulfide signaling in plants.
- The identified S-sulfhydrated proteins represent key targets for H2S-mediated regulation.
- This reversible modification process in plants mirrors findings in mammalian systems, highlighting conserved signaling pathways.
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