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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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Updated: Apr 15, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Decoding viral infection by ribosome profiling.

Noam Stern-Ginossar¹

¹Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel noam.stern-ginossar@weizmann.ac.il.

Journal of Virology

|March 27, 2015

Summary

This summary is machine-generated.

Ribosome profiling, a deep sequencing method, reveals new details about protein production and translation regulation in cells. This technique uncovered unexpected complexity in herpesvirus coding capacity during infection.

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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Area of Science:

Molecular Biology
Virology
Genomics

Background:

Ribosome profiling is an advanced deep sequencing technique for monitoring cellular translation.
Previous studies have utilized ribosome profiling to gain insights into protein synthesis, regulation, and cellular protein production.
Understanding viral gene expression is crucial for deciphering infection mechanisms.

Purpose of the Study:

To discuss the application of ribosome profiling in studying viral infections.
To highlight novel findings regarding herpesvirus coding capacity using ribosome profiling.
To explore the potential of ribosome profiling in uncovering new insights into viral infection processes.

Main Methods:

Ribosome profiling (also known as Ribo-Seq) was employed to analyze translation in infected cells.
Deep sequencing was used to generate high-throughput data on ribosome occupancy.
Comparative analysis of ribosome profiling data from infected and uninfected cells.

Main Results:

Application of ribosome profiling to cells infected with human cytomegalovirus (HCMV) and Kaposi's sarcoma-associated herpesvirus (KSHV) revealed significant complexity in their coding capacity.
Novel insights into the mechanisms of viral protein synthesis and translation regulation were obtained.
Unanticipated viral gene expression patterns were identified.

Conclusions:

Ribosome profiling is a powerful tool for investigating viral gene expression and infection dynamics.
The study demonstrated the utility of ribosome profiling in uncovering previously unknown aspects of viral coding capacity.
Future applications of ribosome profiling in virology promise to yield further discoveries regarding virus-host interactions and infection mechanisms.