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Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

27.4K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
27.4K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

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12.1K
Bacterial Transcription01:53

Bacterial Transcription

39.6K
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
39.6K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

34.0K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
34.0K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

14.3K
14.3K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

19.9K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
19.9K

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Related Experiment Video

Updated: Apr 15, 2026

Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription
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Application of Biolayer Interferometry BLI for Studying Protein-Protein Interactions in Transcription

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Preface. Bacterial transcriptional control

Irina Artsimovitch, Thomas J Santangelo

    Methods in Molecular Biology (Clifton, N.J.)
    |March 27, 2015
    PubMed
    Summary

    No abstract available in PubMed .

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