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Updated: Apr 15, 2026

Accurate Follicle Enumeration in Adult Mouse Ovaries

Accurate Follicle Enumeration in Adult Mouse Ovaries

Published on: October 16, 2020

Computer-generated ovaries to assist follicle counting experiments.

Angelos Skodras¹, Gianluca Marcelli²

¹German Centre for Neurodegenerative Diseases (DZNE), Tübingen, Germany.

|March 27, 2015

Summary

Estimating ovarian follicle numbers in mice is crucial for reproductive biology and toxicology studies. This research introduces a novel computer simulation to optimize experimental design, reducing animal use and improving accuracy.

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Area of Science:

Reproductive Biology
Developmental Biology
Toxicology

Background:

Accurate ovarian follicle counting is vital for understanding reproductive development and assessing environmental/therapeutic impacts.
Estimates are influenced by sampling methods and inherent biological variability within animal populations.
Neonatal mouse ovaries are a key model for studying these factors.

Purpose of the Study:

To assess the impact of sampling methods and biological variability on ovarian follicle number estimation in neonatal mice.
To develop and validate a computational tool for optimizing experimental design in reproductive studies.
To enhance the accuracy and efficiency of follicle counting methodologies.

Main Methods:

Development of computer algorithms to generate simulated neonatal mouse ovaries based on published data.

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Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

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Last Updated: Apr 15, 2026

Accurate Follicle Enumeration in Adult Mouse Ovaries

Accurate Follicle Enumeration in Adult Mouse Ovaries

Published on: October 16, 2020

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Published on: June 19, 2018

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

In-silico counting experiments utilizing unbiased stereological techniques on simulated ovaries.

Modeling of biological variability and sampling strategies to predict experimental outcomes.

Main Results:

The developed computer algorithms successfully model neonatal mouse ovaries with realistic characteristics.
In-silico experiments demonstrate the utility of simulated ovaries for optimizing sampling frequency and animal numbers.
The approach provides data-driven recommendations for achieving desired accuracy in follicle count estimations.

Conclusions:

Simulated ovaries offer a versatile and novel tool for the planning phase of reproductive biology experiments.
This in-silico approach aids in estimating required animal numbers and workload, ensuring adequate statistical power.
The simulated ovary concept holds potential for application to other organs with numerous functional units.