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Related Concept Videos

Arboviral Encephalitis01:25

Arboviral Encephalitis

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Arboviral encephalitis refers to brain inflammation caused by arthropod-borne viruses, particularly those transmitted through mosquito vectors. Among these, West Nile virus (WNV), a member of the Flaviviridae family, is a significant public health concern. WNV is an enveloped, positive-sense, single-stranded RNA virus. Human infection typically begins when an infected mosquito introduces the virus into the dermis during feeding. The primary transmission cycle involves birds as amplifying hosts...
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The equine arteritis virus isolate from the 2010 Argentinian outbreak.

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    Equine arteritis virus (EAV) strains from Argentina were analyzed, revealing a new strain belonging to the European group. This highlights the need for monitoring infected stallions and semen to prevent EAV persistence in horse populations.

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    Area of Science:

    • Veterinary Virology
    • Molecular Epidemiology
    • Equine Infectious Diseases

    Background:

    • Equine arteritis virus (EAV) causes significant disease in horses worldwide.
    • Persistently infected stallions serve as a crucial reservoir for EAV transmission.
    • Understanding viral genetic diversity is key to controlling outbreaks.

    Purpose of the Study:

    • To isolate and characterize a novel EAV strain from an infected stallion in Argentina.
    • To determine the phylogenetic relationship of the new strain with existing EAV isolates.
    • To assess the implications for EAV persistence and control strategies.

    Main Methods:

    • Viral isolation from stallion semen followed by immunofluorescence identification.
    • Reverse-transcription polymerase chain reaction (RT-PCR) amplification of the GP5 gene.
    • DNA sequencing, phylogenetic analysis using neighbor-joining, and N-glycosylation prediction.

    Main Results:

    • A new EAV strain (GLD-LP-ARG) was identified and sequenced.
    • Phylogenetic analysis placed the new strain within the European EAV group (EU-1).
    • High nucleotide identity (99%) was observed with strain Al1 and 98.1% with strain 08P178.

    Conclusions:

    • Persistently infected stallions and their semen are significant reservoirs for EAV.
    • The findings underscore the importance of continuous monitoring of stallions and semen.
    • RT-PCR and test mating are vital tools for EAV control in accordance with regulations.