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Comparison of biomarkers between PLA2RAb+ and PLA2RAb- in patients with idiopathic membranous nephropathy
Chunhua Xun1, Lihua shuai, Wenjuan Wang
1Laboratory Department, The Affiliated Hospital of Jiu Jiang University, Jiujiang, 332000, Jiangxi, China, z6x2m@163.com.
Objective:
The presence of antibodies against the M-type phospholipase A2 receptor (PLA2RAb) is considered to be a promising serological diagnostic biomarker of idiopathic membranous nephropathy (IMN). We compare the change in serum Cystain C (CysC), urea, creatinine (CREA), uric acid (UA), total protein (TP), albumin (ALB), IgG4 and 24-h urinary protein (proteinuria, PRO) between PLA2RAb+ and PLA2RAb- of IMN patients.
Materials And Methods:
The serum and urine samples were collected from 120 patients with IMN. The presence of circulating PLA2RAb was determined by indirect immunofluorescence, and their titer was quantified by ELISA. CysC, urea, CREA, UA, TP, ALB and 24-h PRO were examined by automatic biochemical analyzer.
Results:
Serum IgG4 level was determined by specific protein analyzer. PLA2RAb-positive percentage by ELISA was higher than that by IIF, but no significant difference was found by McNemar's Chi-square test. Serum IgG4 level and 24-h PRO level were significantly higher in PLA2RAb+ than in PLA2RAb- (P < 0.05). In PLA2RAb+ group, PLA2RAb is positively related to serum IgG4 and 24-h PRO and negatively related to serum TP and ALB (P < 0.01).
Conclusion:
This is the first study to show that combined detection of IgG4 concentration and PLA2RAb was usable for IMN patients.
