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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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A Rapid In Vivo Bioassay for Developmentally Active Enhancers
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Brg1 modulates enhancer activation in mesoderm lineage commitment.

Jeffrey M Alexander1, Swetansu K Hota1, Daniel He1

  • 1Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA Roddenberry Center for Stem Cell Biology and Medicine at Gladstone, San Francisco, CA 94158, USA.

Development (Cambridge, England)
|March 28, 2015
PubMed
Summary
This summary is machine-generated.

Chromatin remodeling factor Brg1 is crucial for activating enhancers during mesoderm induction. Its absence disrupts cardiomyocyte differentiation and gene regulation, highlighting Brg1

Keywords:
ChromatinEnhancersGene expressionHistone modificationMesodermStem cells

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Area of Science:

  • Developmental Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Understanding gene regulation during development is key.
  • Histone modifications and chromatin remodeling roles are unclear.

Purpose of the Study:

  • Investigate Brg1's role in mesoderm induction.
  • Elucidate Brg1's function in enhancer activation and lineage commitment.

Main Methods:

  • Embryonic stem cell directed differentiation assay.
  • Analysis of Brg1's co-localization with H3K27ac at enhancers.
  • Assessment of Polycomb-mediated repression maintenance.

Main Results:

  • Brg1 absence prevents cardiomyocyte differentiation and deregulates gene expression.
  • Brg1 co-localizes with H3K27ac and is required for enhancer acetylation.
  • Brg1 maintains Polycomb repression of non-mesodermal genes.

Conclusions:

  • Brg1 is essential for activating enhancers during mesoderm induction.
  • Brg1 modulates active and repressive chromatin states for lineage commitment.
  • Interplay between chromatin remodelers and histone modifications ensures robust gene regulation.