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Updated: Apr 15, 2026
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Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
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3-nitrotyrosine modified proteins in atherosclerosis
1Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.
Disease Markers
|March 28, 2015
Summary
Atherosclerosis involves foam cells and oxidative stress. Modified proteins, specifically nitrotyrosine, correlate with cardiovascular disease prevalence, indicating its potential as a predictive biomarker.
Area of Science:
- Cardiovascular research
- Atherosclerosis
- Oxidative stress biology
Background:
- Cardiovascular disease (CVD) is a major cause of global mortality, with atherosclerosis as its primary driver.
- Atherosclerosis is characterized by the accumulation of lipid-laden macrophages (foam cells) and chronic inflammation.
- Oxidative posttranslational modifications, such as protein nitration, occur in atherosclerotic lesions and plasma.
Purpose of the Study:
- To investigate the role of nitrotyrosine modified proteins in atherosclerosis.
- To assess the potential of nitrotyrosine as a biomarker for cardiovascular disease.
Main Methods:
- Analysis of atherosclerotic lesions and plasma from patients.
- Identification and quantification of nitrotyrosine modified proteins.
Main Results:
- Nitrotyrosine modified proteins were detected in atherosclerotic lesions and plasma.
- A significant correlation was observed between plasma protein nitration levels and cardiovascular disease prevalence.
Conclusions:
- Protein nitration, specifically 3-nitrotyrosine, is linked to proatherogenic and prothrombotic properties.
- Nitrotyrosine serves as a valuable biomarker for predicting cardiovascular disease outcomes and guiding therapeutic decisions.
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