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Updated: Apr 15, 2026

Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
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Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

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3-nitrotyrosine modified proteins in atherosclerosis

Leonor Thomson1

  • 1Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.

Disease Markers
|March 28, 2015
PubMed

Insights

Atherosclerosis involves foam cells and oxidative stress. Modified proteins, specifically nitrotyrosine, correlate with cardiovascular disease prevalence, indicating its potential as a predictive biomarker.

Area of Science:

  • Cardiovascular research
  • Atherosclerosis
  • Oxidative stress biology

Background:

  • Cardiovascular disease (CVD) is a major cause of global mortality, with atherosclerosis as its primary driver.
  • Atherosclerosis is characterized by the accumulation of lipid-laden macrophages (foam cells) and chronic inflammation.
  • Oxidative posttranslational modifications, such as protein nitration, occur in atherosclerotic lesions and plasma.

Purpose of the Study:

  • To investigate the role of nitrotyrosine modified proteins in atherosclerosis.
  • To assess the potential of nitrotyrosine as a biomarker for cardiovascular disease.

Main Methods:

  • Analysis of atherosclerotic lesions and plasma from patients.
  • Identification and quantification of nitrotyrosine modified proteins.

Main Results:

  • Nitrotyrosine modified proteins were detected in atherosclerotic lesions and plasma.
  • A significant correlation was observed between plasma protein nitration levels and cardiovascular disease prevalence.

Conclusions:

  • Protein nitration, specifically 3-nitrotyrosine, is linked to proatherogenic and prothrombotic properties.
  • Nitrotyrosine serves as a valuable biomarker for predicting cardiovascular disease outcomes and guiding therapeutic decisions.

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