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Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

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Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging

Author Spotlight: In-Depth Morphometric Examination and Quantification of Native Lens Structure Using Whole Mount Imaging

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Lens capsule structure assessed with atomic force microscopy.

Vivian M Sueiras¹, Vincent T Moy², Noël M Ziebarth¹

¹Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, FL.

Molecular Vision

|March 28, 2015

Summary

This summary is machine-generated.

Atomic force microscopy (AFM) revealed nanoscale fibrous structures on the anterior lens capsule across species. Human lens capsule spacing decreased with age, and species-specific differences in surface roughness were observed.

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Area of Science:

Ophthalmology
Biomaterials Science
Nanotechnology

Background:

The anterior lens capsule provides structural integrity to the lens.
Understanding its nanoscale architecture is crucial for comprehending lens biomechanics and age-related changes.

Purpose of the Study:

To utilize atomic force microscopy (AFM) for high-resolution imaging of the anterior lens capsule's ultrastructure at the nanoscale.
To investigate species-dependent variations in lens capsule nanostructure and surface properties.

Main Methods:

Anterior lens capsules from human, cynomolgus monkey, and porcine eyes were analyzed using AFM in contact mode.
Interfibrillar spacing, fiber diameter, and surface roughness were quantified using integrated analysis software.
Sample hydration was maintained using Dulbecco's Modified Eagle Medium (DMEM).

Main Results:

AFM imaging demonstrated a consistent, highly ordered fibrous nanostructure on the anterior lens capsule in all species studied.
Significant differences in interfibrillar spacing were found: porcine (0.68±0.25 μm), human (1.08±0.25 μm), and cynomolgus monkey (1.80±0.39 μm).
Primate lens capsule interfibrillar spacing showed a linear decrease with increasing age, and surface roughness varied significantly between species, with porcine being the smoothest.

Conclusions:

Atomic force microscopy (AFM) effectively imaged the nanostructure of the anterior lens capsule.
The study identified distinct species-dependent differences in the nanostructure and surface roughness of the lens capsule.
Age-related changes in interfibrillar spacing were observed in primate lens capsules.