Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

17.9K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
17.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation.

Signal transduction and targeted therapy·2026
Same author

Engineering microcavity arrays as a niche for high-throughput and high-content analysis of tumoroids: A detailed methodology.

Methods in cell biology·2026
Same author

Single-cell viability assessment using YOLOv8n object detection.

Methods in cell biology·2026
Same author

Datopotamab deruxtecan induces hallmarks of immunogenic cell death.

Cell stress·2025
Same author

Acyl-CoA-binding protein as a driver of pathological aging.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Lysosomal membrane permeabilization enhances the anticancer effects of POLR1 (RNA polymerase I) transcription inhibitors.

Autophagy·2025

Related Experiment Video

Updated: Apr 15, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.6K

Quantification of cellular viability by automated microscopy and flow cytometry.

Allan Sauvat1,2,3, Yidan Wang1,2,3,4, Florian Segura1,2,3

  • 1Equipe 11 labellisée par la Ligue Nationale contre le Cancer, Centre de Recherche des Cordeliers, Paris, France.

Oncotarget
|March 28, 2015
PubMed
Summary

This study introduces a new method to accurately identify dead, dying, and healthy cells by simultaneously assessing plasma membrane integrity, mitochondrial function, and caspase activation. This advanced cell viability assay improves cell death detection in research and high-throughput screening.

Keywords:
apoptosisdrug discoveryhigh-throughput screeningnecrosis

More Related Videos

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

49.6K
An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells
09:58

An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells

Published on: August 29, 2025

1.1K

Related Experiment Videos

Last Updated: Apr 15, 2026

Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

47.6K
Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

49.6K
An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells
09:58

An Optimized LIVE/DEAD Assay Coupled with Flow Cytometry for Quantifying Post-Stress Survival in Yeast Cells

Published on: August 29, 2025

1.1K

Area of Science:

  • Cell Biology
  • Biotechnology
  • Biochemistry

Background:

  • Traditional cell viability assays using dyes like 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33342 may misclassify cells committed to death.
  • Cells excluding DAPI or showing normal nuclear morphology can still have lost mitochondrial function or activated caspases, indicating irreversible cell death.

Purpose of the Study:

  • To develop a robust protocol for simultaneous detection of multiple cell death indicators.
  • To enable precise quantification of healthy, dying, and dead cells using a single, integrated assay.

Main Methods:

  • Developed a protocol combining DAPI/Hoechst 33342 for membrane integrity/nuclear morphology, DiOC6(3) for mitochondrial membrane potential, and YO-PRO®-3 for caspase activation.
  • Utilized epifluorescence microscopy and flow cytometry for analysis.
  • Ensured compatibility with robotized, high-throughput workflows.

Main Results:

  • Successfully developed a multi-parameter assay for simultaneous assessment of cell viability indicators.
  • Enabled precise quantification of cell populations based on membrane integrity, mitochondrial function, and caspase activity.
  • Validated the method for use in high-throughput screening platforms.

Conclusions:

  • The novel protocol offers a more accurate assessment of cellular viability than traditional methods.
  • This assay precisely quantifies cell death stages, improving the reliability of cell viability studies.
  • The method is adaptable to various platforms, including high-throughput screening, advancing cell death research.