Related Experiment Video
Updated: Apr 15, 2026
04:45
An Epithelial Abrasion Model for Studying Corneal Wound Healing
Published on: December 29, 2021
4.0K
Thrombomodulin promotes corneal epithelial wound healing
Yi-Hsun Huang1, Ching-Chang I2, Cheng-Hsiang Kuo2
1Institute of Clinical Medicine, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Plos One
|March 28, 2015
Summary
Thrombomodulin (TM) plays a role in corneal wound healing and is regulated by platelet-derived growth factor-BB (PDGF-BB). Recombinant TM epidermal growth factor-like domain plus serine/threonine-rich domain (rTMD23) accelerates corneal epithelial wound healing.
Area of Science:
- Ophthalmology
- Regenerative Medicine
Background:
- Thrombomodulin (TM) is a crucial protein involved in various biological processes.
- Its role in corneal epithelial wound healing requires further elucidation.
Purpose of the Study:
- To determine the function of TM in corneal epithelial wound healing.
- To investigate the therapeutic potential of recombinant TM epidermal growth factor-like domain plus serine/threonine-rich domain (rTMD23) for corneal wound repair.
Main Methods:
- Immunofluorescence staining to assess TM localization and expression in murine corneas.
- In vitro and in vivo assays to evaluate the efficacy of rTMD23 in corneal wound healing.
- Investigated the signaling pathway (mTOR/Ets-1) involved in PDGF-BB-induced TM expression.
Main Results:
- TM is expressed in normal adult murine corneas, with increased expression during early wound healing.
- Platelet-derived growth factor-BB (PDGF-BB) upregulates TM expression in corneal epithelial cells via the mTOR/Ets-1 pathway.
- Administration of rTMD23 significantly enhanced the rate of corneal epithelial wound healing.
Conclusions:
- Corneal epithelial TM expression is modulated during wound healing, potentially regulated by PDGF-BB.
- rTMD23 demonstrates therapeutic potential for accelerating corneal epithelial wound healing and treating corneal injuries.
Related Concept Videos
01:16Clot Retraction and Fibrinolysis
10.5K
After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
10.5K
01:28Phases of Wound Repair
9.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
9.9K
01:22Formation of the Platelet Plug
11.0K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
11.0K
01:20Extrinsic and Intrinsic Pathways of Hemostasis
15.9K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
15.9K
01:28Tissue Injury: Inflammation and Repair
5.9K
5.9K
01:24Inflammatory Response II: Inflammatory Exudate and Tissue Repair
9.6K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
9.6K