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[A method for determination of Neisseria meningitidis serogroup A, B, C and W by real-time PCR]

K O Mironov, A E Platonov, O P Dribnokhodova

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |March 31, 2015
    PubMed
    Abstract

    Insights

    A new real-time PCR method accurately identifies Neisseria meningitidis DNA from cerebrospinal fluid (CSF) samples. This rapid diagnostic tool effectively serogroups common meningococcal strains, aiding in meningitis diagnosis.

    Area of Science:

    • Molecular Biology
    • Microbiology
    • Infectious Diseases

    Background:

    • Neisseria meningitidis causes bacterial meningitis, a severe infection.
    • Accurate and rapid serogroup identification is crucial for effective treatment and public health response.
    • Existing diagnostic methods may have limitations in speed and specificity.

    Purpose of the Study:

    • To develop and validate a real-time PCR assay for detecting Neisseria meningitidis DNA.
    • To enable simultaneous identification of serogroups A, B, C, and W.
    • To assess the method's performance on clinical cerebrospinal fluid (CSF) samples.

    Main Methods:

    • Development of multiplex real-time PCR assays targeting specific Neisseria meningitidis serogroup loci.
    • Optimization of PCR conditions for specificity and analytical sensitivity.
    • Testing on reference strains and 187 clinical CSF samples from patients with meningococcal meningitis.

    Main Results:

    • The real-time PCR method successfully detected Neisseria meningitidis DNA in clinical samples.
    • Serogroup distribution in CSF samples: A (55%), B (24%), C (16%), W (3%).
    • The assay demonstrated high specificity and sensitivity, with potential for identifying other serogroups.

    Conclusions:

    • The developed real-time PCR method can serogroup at least 95% of Neisseria meningitidis strains or DNA from CSF.
    • This method, combined with genotyping techniques, is valuable for comprehensive characterization of pathogenic Neisseria meningitidis.

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