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Related Experiment Videos

Cell volume regulation in frog urinary bladder.

C W Davis, A L Finn

    Federation Proceedings
    |June 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Toad and frog urinary bladder cells regulate volume by losing potassium, chloride, and water after swelling. Calcium is crucial for this cell volume homeostasis, controlling potassium permeability and membrane interactions.

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    Area of Science:

    • Cell biology
    • Physiology
    • Membrane transport

    Background:

    • Cell volume homeostasis is critical for cellular function.
    • Urinary bladders maintain osmotic balance through regulated ion and water transport.
    • Amiloride-sensitive sodium channels play a key role in epithelial transport.

    Purpose of the Study:

    • To investigate the mechanisms of cell volume regulation in amphibian urinary bladders.
    • To elucidate the role of ion transport and calcium in maintaining cell volume homeostasis.
    • To understand the interplay between apical and basolateral membrane conductances during volume regulation.

    Main Methods:

    • Electrophysiological measurements to assess membrane potentials and resistances.
    • Optical methods to measure cell volume changes.

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  • Pharmacological manipulation using amiloride and calcium ionophores (A23187).

    • Main Results:

    • Osmotically swollen cells spontaneously regulate volume via K+, Cl-, and water loss.
    • Amiloride inhibits volume regulation by blocking apical sodium entry and increasing basolateral resistance.
    • Calcium is essential for regulating basolateral potassium permeability and volume recovery.

    Conclusions:

    • Inhibition of apical sodium transport leads to decreased basolateral potassium permeability.
    • Calcium acts as an intracellular mediator controlling basolateral potassium channels.
    • Calcium is indispensable for effective cell volume regulation in response to osmotic stress.