Related Experiment Video
Updated: Apr 15, 2026
10:21
Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
45.3K
Systematic and practical solvent system selection strategy based on the nonrandom two-liquid segment activity
Da-Bing Ren1, Lun-Zhao Yi1, Yan-Hua Qin2
1Yunnan Food Safety Research Institute, Kunming University of Science and Technology, Kunming 650500, China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Journal of Chromatography. A
|March 31, 2015
Summary
A new strategy using the nonrandom two-liquid segment activity coefficient (NRTL-SAC) model efficiently predicts solute partition coefficients for counter-current chromatography (CCC) solvent system selection. This method accelerates the screening process for effective separations.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Solvent system selection is critical for successful counter-current chromatography (CCC) separations.
- Predicting solute partition coefficients is essential for optimizing CCC solvent systems.
Purpose of the Study:
- To introduce a systematic solvent system selection strategy based on the nonrandom two-liquid segment activity coefficient (NRTL-SAC) model.
- To extend and validate the NRTL-SAC method for predicting solute partition coefficients in various solvent systems for CCC.
Main Methods:
- Application of the NRTL-SAC model to ethyl acetate/n-butanol/water and chloroform/methanol/water solvent systems.
- Investigation of the NRTL-SAC method's versatility and predictive capability across different solvent families.
- Validation of the NRTL-SAC strategy through the separation of five components from Salvia plebeian R.Br.
Main Results:
- The NRTL-SAC model efficiently predicts solute partition coefficients.
- Solute molecular parameters derived from one solvent system (hexane/ethyl acetate/methanol/water) accurately predicted coefficients in other solvent systems.
- Successful separation of five components from Salvia plebeian R.Br. using the NRTL-SAC guided solvent selection.
Conclusions:
- The NRTL-SAC model provides a promising, high-throughput method for rapid solvent system selection in CCC.
- The NRTL-SAC strategy is highly adaptable for screening suitable solvent systems for real-world CCC applications.
Related Concept Videos
01:15Optimizing Chromatographic Separations
1.3K
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
1.3K
01:09Analyte Adsorption and Distribution
3.3K
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
3.3K
01:05High-Performance Liquid Chromatography: Elution Process
2.1K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.1K
01:11Thin-Layer Chromatography (TLC): Overview
6.8K
Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
6.8K
01:11High-Performance Liquid Chromatography: Introduction
4.3K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
4.3K
01:10Silica Gel Column Chromatography: Overview
4.4K
Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
4.4K