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Updated: Apr 15, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
DNA interaction, SOD, peroxidase and nuclease activity studies of iron complex having ligand with carboxamido
Kaushik Ghosh1, Nidhi Tyagi1, Hemant Kumar1
1Department of Chemistry, Indian Institute of Technology, Roorkee, Roorkee 247667, Uttarakhand, India.
Abstract:
Complex (Et3HN)[Fe(III)(bpb)Cl2], 1 {where H2bpb: N,N'-(1,2-phenylene)bis(pyridine-2-carboxamide)} was synthesized and characterized by reported procedure (Yang et al., 1991). Complex 1 was found to be effective in superoxide scavenging activity and an IC50 value of 4.1 μM was obtained in xanthine-xanthine oxidase nitro blue tetrazolium assay. Peroxidase-like activity of this complex was determined by the oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS). DNA interaction studies of complex 1 showed binding of DNA through external or groove binding. Complex 1 exhibited chemical nuclease activity in the presence of hydrogen peroxide and cleaved supercoiled pBR322 DNA to its linear and nicked circular form at physiological pH. Mechanistic studies indicated possible role of hydroxyl radical (·OH) species in DNA cleavage activity via hydroperoxo intermediate: [Fe(III)OOH(-)](2+)→[Fe(IV)O](2+)+(·)OH.
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