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Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Innovative Microneedle-Based Strategies for Enhanced Exosome Delivery and Stability
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Feasibility study for intraepidermal delivery of proteins using a solid microneedle array.

Madeleine Witting1, Katja Obst2, Markus Pietzsch3

  • 1Department of Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians-Universität Munich, Germany.

International Journal of Pharmaceutics
|March 31, 2015
PubMed
Summary

Solid microneedles (MN) offer a stable, pain-free method for delivering proteins intradermally. This study shows MN can deliver proteins with high bioactivity and stability for up to three months.

Keywords:
Coating formulationHuman reconstructed skinProtein stabilityProteinsSkin irritationSolid microneedle array

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Area of Science:

  • Biomaterials Science
  • Dermal Drug Delivery
  • Vaccination Technology

Background:

  • Solid microneedles (MN) are explored for painless, safe, and patient-friendly dermal antigen delivery, particularly for vaccines.
  • Existing research focuses on coating techniques and formulations for vaccine stability and targeted skin deposition.
  • Limited data exists on the process and long-term storage stability of coated MN.

Purpose of the Study:

  • To assess the feasibility of solid MN for intraepidermal protein delivery.
  • To develop a protein-stabilizing coating formulation for MN.
  • To evaluate the stability and deposition efficiency of coated MN.

Main Methods:

  • Determined optimal needle length (300 μm) for intraepidermal protein delivery.
  • Developed a protein-stabilizing coating formulation for MN.
  • Quantified protein concentration and bioactivity post-coating and storage.
  • Assessed protein deposition in skin via MN application.

Main Results:

  • Coated MN contained 10–23 μg of protein.
  • 90% of model protein (asparaginase) bioactivity was retained for 3 months.
  • 68.0 ± 11.7% of coated protein was deposited intraepidermally after one application.
  • Minor skin irritation (increased interleukin 8) observed post-insertion.

Conclusions:

  • Solid MN are a feasible approach for selective intraepidermal deposition of proteins and peptides.
  • Protein stabilization during storage is a key advantage demonstrated in this study.
  • MN technology shows promise for advanced dermal delivery systems, including vaccination.