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Updated: Apr 15, 2026

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses
Published on: June 8, 2022
Pannexin 1 facilitates arterial relaxation via an endothelium-derived hyperpolarization mechanism
Dina Gaynullina1, Valery I Shestopalov2, Yury Panchin3
1State Research Center of the Russian Federation - Institute for Biomedical Problems RAS, Khoroshevskoe shosse 76A, 123007 Moscow, Russia; Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia; Department of Physiology, Russian National Research Medical University, Ostrovityanova Str. 1, 117997 Moscow, Russia.
Abstract:
Pannexin 1 (Panx1) is involved in endothelium-dependent vasodilation in large arteries, but the exact mechanistic role remains poorly understood. We hypothesized that Panx1 facilitates large vessel relaxations regulating endothelium-derived hyperpolarization (EDH)-like mechanisms. The EDH-like component of acetylcholine-induced relaxation of saphenous arteries studied in isometric myograph after inhibition of NO-synthase and cyclooxygenase was significantly impaired in mice with genetically ablated Panx1 (KO) relative to that in the wild type (WT) mice. Application of P1-receptor antagonist and apyrase significantly reduced this component in WT, but not in KO mice, indicating participation of ATP released via Panx1 in the EDH-like relaxation.
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