Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mouse Tumor Tissue-Derived Extracellular Vesicles Induce Angiogenesis Through VEGF Production From Macrophages.

Journal of extracellular vesicles·2025

Same author

Metabolic Reprogramming Into a Glycolysis Phenotype Induced by Extracellular Vesicles Derived From Prostate Cancer Cells.

Molecular & cellular proteomics : MCP·2025

Same author

Mass-produced gram-negative bacterial outer membrane vesicles activate cancer antigen-specific stem-like CD8<sup>+</sup> T cells which enables an effective combination immunotherapy with anti-PD-1.

Journal of extracellular vesicles·2023

Same author

Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins.

Journal of extracellular vesicles·2023

Same author

Efficient Labeling of Vesicles with Lipophilic Fluorescent Dyes via the Salt-Change Method.

Analytical chemistry·2023

Same author

Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice.

International journal of biological sciences·2022

Search research articles

Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

Isolation of extracellular vesicles for proteomic profiling.

Dong-Sic Choi¹, Yong Song Gho

¹Department of Life Sciences, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Republic of Korea.

Methods in Molecular Biology (Clifton, N.J.)

|March 31, 2015

Summary

Extracellular vesicles (EVs) are key in cell communication and disease. This study details isolating EVs from SW480 cells and preparing them for proteomic analysis using mass spectrometry.

More Related Videos

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

Related Experiment Videos

Last Updated: Apr 15, 2026

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Author Spotlight: Effective Reuse of Polycarbonate Tubes for Extracellular Vesicle Isolation

Published on: March 8, 2024

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

Area of Science:

Cell Biology
Biochemistry
Biotechnology

Background:

Extracellular vesicles (EVs) are nano-sized vesicles involved in intercellular communication, transferring proteins, DNA, and RNA.
EVs contain biomarkers reflecting cellular status, making them valuable for diagnostics and therapeutics.
Current challenges exist in isolating EVs for comprehensive analysis.

Purpose of the Study:

To describe a method for isolating extracellular vesicles from SW480 cells.
To outline the preparation of tryptic peptides from isolated EVs for proteomic analysis.
To highlight the potential of EVs as diagnostic biomarkers and therapeutic targets.

Main Methods:

Isolation of extracellular vesicles from SW480 cell cultures.
Purification of EVs using density gradient ultracentrifugation.
Preparation of tryptic peptides for mass spectrometry-based proteomic analysis.

Main Results:

Successful isolation of extracellular vesicles from SW480 cells.
Preparation of EV-derived tryptic peptides suitable for proteomic profiling.
Demonstration of a viable workflow for EV proteome analysis.

Conclusions:

Density gradient ultracentrifugation is a promising method for EV isolation.
Proteomic analysis of EVs can reveal cell- or disease-specific biomarkers.
This methodology supports further research into EV functions and applications.