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Updated: Apr 15, 2026
Chronic Transcranial Electrical Stimulation and Intracortical Recording in Rats
Published on: May 11, 2018
Proteomic study of retinal proteins associated with transcorneal electric stimulation in rats
Takashi Kanamoto1, Nazariy Souchelnytskyi2, Takuji Kurimoto3
1Department of Ophtahlomology, Hiroshima Memorial Hospital, 1-4-3 Honkawacho, Minami-ku, Hiroshima 730-0802, Japan.
Abstract:
Background. To investigate how transcorneal electric stimulation (TES) affects the retina, by identifying those proteins up- and downregulated by transcorneal electric stimulation (TES) in the retina of rats. Methods. Adult Wistar rats received TES on the left eyes at different electrical currents while the right eyes received no treatment and served as controls. After TES, the eye was enucleated and the retina was isolated. The retinas were analyzed by proteomics. Results. Proteomics showed that twenty-five proteins were upregulated by TES. The identified proteins included cellular signaling proteins, proteins associated with neuronal transmission, metabolic proteins, immunological factors, and structural proteins. Conclusions. TES induced changes in expression of various functional proteins in the retina.
Insights
Transcorneal electrical stimulation (TES) alters retinal protein expression in rats. This study identified 25 upregulated proteins, including those involved in cell signaling, neuronal transmission, metabolism, immunity, and structure.
Area of Science:
- Ophthalmology
- Neuroscience
- Proteomics
Background:
- Transcorneal electrical stimulation (TES) is a technique with potential therapeutic applications.
- The precise molecular effects of TES on the retina remain incompletely understood.
Purpose of the Study:
- To investigate the impact of TES on the rat retina.
- To identify proteins that are upregulated or downregulated by TES in the retina using proteomics.
Main Methods:
- Adult Wistar rats underwent TES in one eye, with the contralateral eye serving as a control.
- Retinal tissue was isolated after TES for proteomic analysis.
Main Results:
- Proteomic analysis revealed 25 proteins upregulated by TES.
- The identified proteins encompass diverse functional categories, including cellular signaling, neuronal transmission, metabolism, immunology, and structural components.
Conclusions:
- TES induces significant changes in the expression of various functional proteins within the retina.
- These findings provide insights into the molecular mechanisms underlying TES effects on retinal tissue.
