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Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.8K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
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  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Irx1 Hypomethylation Promotes Osteosarcoma Metastasis Via Induction Of Cxcl14/nf-κb Signaling.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Irx1 Hypomethylation Promotes Osteosarcoma Metastasis Via Induction Of Cxcl14/nf-κb Signaling.

Related Experiment Video

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

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IRX1 hypomethylation promotes osteosarcoma metastasis via induction of CXCL14/NF-κB signaling.

Jinchang Lu, Guohui Song, Qinglian Tang

    The Journal of Clinical Investigation
    |March 31, 2015

    View abstract on PubMed

    Summary
    This summary is machine-generated.

    Epigenetic changes in the IRX1 gene promote osteosarcoma metastasis to the lungs. IRX1 hypomethylation in circulating tumor DNA may predict lung metastasis risk in patients.

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    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
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    LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

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    Area of Science:

    • Oncology
    • Epigenetics
    • Molecular Biology

    Background:

    • Osteosarcoma is a bone cancer that frequently metastasizes to the lungs.
    • Epigenetic alterations are implicated in osteosarcoma development, but their role in metastasis is unclear.

    Purpose of the Study:

    • To investigate epigenetic mechanisms driving osteosarcoma metastasis.
    • To identify novel metastasis-associated genes and their regulatory pathways.

    Main Methods:

    • Analysis of two human osteosarcoma cell lines with different metastatic potentials.
    • Methylated DNA immunoprecipitation (MeDIP) and microarray expression analysis.
    • In vitro and in vivo functional assays in osteosarcoma models.
    • Analysis of circulating tumor DNA (ctDNA) from osteosarcoma patients.

    Main Results:

    • Identified Iroquois homeobox 1 (IRX1) as a metastasis-associated gene.
    • IRX1 overexpression correlated with promoter hypomethylation in cell lines and patient tissues.
    • Modulating IRX1 affected cell migration, invasion, anoikis resistance, and lung metastasis.
    • IRX1 promoted metastasis via CXCL14/NF-κB signaling.
    • IRX1 promoter hypomethylation in ctDNA predicted reduced lung metastasis-free survival.

    Conclusions:

    • IRX1 is a prometastatic gene in osteosarcoma.
    • IRX1 hypomethylation is a potential biomarker for lung metastasis.
    • Targeting epigenetic regulation of IRX1 may offer therapeutic strategies for osteosarcoma metastasis.