Complex of rutin with β-cyclodextrin as potential delivery system

Area of Science:

• Pharmaceutical Sciences
• Materials Science
• Physical Chemistry

Background:

• Rutin, a flavonoid, exhibits beneficial properties but suffers from poor solubility and stability.
• Beta-cyclodextrin (β-CD) is a cyclic oligosaccharide known for its ability to form inclusion complexes.
• Complexation with β-CD offers a strategy to improve the physicochemical and biological properties of poorly soluble drugs.

Purpose of the Study:

• To synthesize and characterize a rutin-beta-cyclodextrin (RU-β-CD) complex.
• To evaluate the impact of complexation on rutin's solubility, stability, antioxidant activity, antibacterial potency, and permeability.
• To investigate the release profile and potential for prolonged drug delivery.

Main Methods:

• Co-grinding technique for complex formation.
• Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM), Fourier-Transform Infrared (FT-IR) spectroscopy, and Raman spectroscopy for characterization.
• Molecular modeling for geometric assessment.
• Electron Paramagnetic Resonance (EPR) spectroscopy to assess antioxidant activity.
• In vitro permeability studies.

Main Results:

• Successful formation of the RU-β-CD complex confirmed by multiple analytical techniques.
• Significantly enhanced solubility and stability of complexed rutin compared to free rutin.
• Slightly reduced antibacterial activity but preserved antioxidant capacity.
• Demonstrated prolonged release of rutin from the complex over 20 hours.
• Achieved approximately double the permeability for complexed rutin versus free rutin.

Conclusions:

• Complexation of rutin with beta-cyclodextrin via co-grinding is an effective strategy to improve its solubility and stability.
• The RU-β-CD complex shows potential for enhanced drug delivery due to improved permeability and sustained release.
• While antibacterial activity is slightly reduced, the antioxidant properties are maintained, and overall therapeutic potential is increased.