Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ionic currents in neuroblastoma clone E-7 cells.

L Vyklický, J Michl, V Vlachová

    Neuroscience Letters
    |April 9, 1985
    PubMed
    Summary

    Differentiated neuroblastoma cells exhibit calcium and potassium currents, while undifferentiated cells only show potassium currents. This study used patch-clamp electrophysiology to investigate these ionic differences.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    From Frog Muscle to Brain Neurons: Joys and Sorrows in Neuroscience.

    Physiological research·2024
    Same author

    Disease-Associated Variants in GRIN1, GRIN2A and GRIN2B genes: Insights into NMDA Receptor Structure, Function, and Pathophysiology.

    Physiological research·2024
    Same author

    Transient receptor potential ankyrin 1 channel: An evolutionarily tuned thermosensor.

    Physiological research·2021
    Same author

    Prof. Arnošt Gutmann 16. 7. 1910 - 6. 8. 1977.

    Physiological research·2019
    Same author

    Nanoscale Spin Manipulation with Pulsed Magnetic Gradient Fields from a Hard Disc Drive Writer.

    Nano letters·2018
    Same author

    Molecular basis of TRPA1 regulation in nociceptive neurons. A review.

    Physiological research·2017

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Electrophysiology

    Background:

    • Neuroblastoma cells are a model for neuronal development.
    • Ionic currents play a crucial role in neuronal function and differentiation.

    Purpose of the Study:

    • To investigate the presence and characteristics of ionic currents in differentiated and undifferentiated mouse neuroblastoma cells.
    • To identify the types of ion channels present in these distinct cell populations.

    Main Methods:

    • Utilized the patch-clamp technique in whole-cell mode.
    • Applied depolarizing voltage steps to neuroblastoma cell cultures.
    • Tested the effects of tetrodotoxin, cobalt ions (Co2+), and tetraethylammonium on ionic currents.

    Main Results:

    • Differentiated cells displayed an early, inactivating inward current (TTX-insensitive, Co2+-sensitive), indicative of Ca2+ channels.
    • A delayed outward current, sensitive to tetraethylammonium (K+ channel blocker), was observed in both cell types.
    • Undifferentiated cells exclusively showed the delayed outward K+ current.

    Conclusions:

    • Differentiated neuroblastoma cells possess both Ca2+ and delayed rectifier K+ channels.
    • Undifferentiated neuroblastoma cells appear to express only delayed rectifier K+ channels.
    • These findings highlight distinct ion channel expression patterns during neuroblastoma cell differentiation.

    Related Experiment Videos