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Updated: Apr 15, 2026

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

Published on: December 29, 2023

Microparticle source and tissue factor expression in pregnancy.

Amy E Wong¹, Hau C Kwaan, William A Grobman

¹Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA, amy.wong@gmail.com.

Annals of Hematology

|April 1, 2015

Summary

Pregnancy does not alter circulating microparticle (MP) origin or tissue factor (TF) expression. However, labor and delivery increase platelet-derived MPs in maternal circulation.

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Last Updated: Apr 15, 2026

Author Spotlight: High-Sensitivity Tissue Factor Activity Assay for Plasma Diagnosis

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Published on: December 29, 2023

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

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Published on: November 22, 2024

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

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Published on: January 26, 2024

Area of Science:

Obstetrics and Gynecology
Hematology
Reproductive Biology

Background:

Microparticles (MPs) expressing tissue factor (TF) are key activators of blood coagulation.
Understanding the origin and dynamics of maternal MPs during pregnancy and labor is crucial for assessing hemostasis.

Purpose of the Study:

To investigate changes in the origin and composition of circulating maternal MPs during pregnancy, labor, and delivery.
To determine if MPs from platelets or trophoblasts contribute to coagulation alterations in pregnancy.

Main Methods:

Prospective cohort study involving non-pregnant, pregnant (non-laboring), and laboring women.
Flow cytometry used to identify platelet-derived, trophoblast-derived, and TF-expressing MPs.
Blood samples collected pre- and post-delivery in pregnant groups.

Main Results:

Pregnancy did not significantly alter MP origin or the number of TF-expressing MPs.
Labor and delivery did not change the percentage of trophoblast-derived MPs.
A significant increase in platelet-derived MPs was observed post-delivery in laboring women.

Conclusions:

Circulating maternal MP origin and TF expression remain stable throughout pregnancy.
Labor and delivery are associated with a notable increase in platelet-derived MPs.
These findings suggest a dynamic shift in MP populations around childbirth.