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Related Experiment Videos

Evidence that receptors mediating central synaptic potentials extend beyond the postsynaptic density.

D S Faber, P G Funch, H Korn

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1985
    PubMed
    Summary

    Synaptic glycine receptors in goldfish Mauthner cells are more widespread than previously thought. This distribution, coupled with restricted diffusion, explains response characteristics, challenging earlier density estimates.

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    Area of Science:

    • Neuroscience
    • Cellular Neuroscience
    • Synaptic Physiology

    Background:

    • The Mauthner cell in goldfish serves as a model for studying synaptic transmission.
    • Glycine is the primary inhibitory neurotransmitter at these synapses.
    • Understanding receptor distribution is crucial for synaptic function.

    Purpose of the Study:

    • To estimate the size and distribution of the postsynaptic receptor matrix at inhibitory synapses on the goldfish Mauthner cell.
    • To investigate the role of glycine receptor density and diffusion in synaptic response kinetics.

    Main Methods:

    • Combined physiological recordings (pre- and postsynaptic) of inhibitory postsynaptic potentials.
    • Computer simulations modeling transmitter diffusion, receptor binding, and channel activation.

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  • Experimental manipulation using strychnine, a glycine receptor antagonist.

    • Main Results:

    • Calculations suggest unrealistically high glycine binding site densities if receptors are confined to the postsynaptic density.
    • Simulations indicate that synaptic receptors are distributed beyond the immediate junctional area.
    • Response rise times appear independent of receptor binding site densities, suggesting a role for restricted diffusion.

    Conclusions:

    • Glycine receptors are widely distributed around inhibitory synapses on the Mauthner cell.
    • A restricted diffusional space surrounding the receptor matrix influences synaptic response kinetics.
    • This geometrical organization reconciles experimental data and challenges previous models of receptor density.