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Microscopic observation of chemical modification in sections using scanning acoustic microscopy
Katsutoshi Miura1, Yuki Egawa2, Toshiaki Moriki2
1Department of Health Science, Pathology and Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Pathology International
|April 1, 2015
Summary
Scanning acoustic microscopy (SAM) measures tissue elasticity by analyzing the speed of sound (SOS). This technique effectively tracks chemical modifications in tissues, offering greater sensitivity than traditional histology.
Area of Science:
- Biophysics
- Materials Science
- Histology
Background:
- Scanning acoustic microscopy (SAM) quantifies the speed of sound (SOS) in tissues, enabling visualization of tissue elasticity.
- Differences in SOS correlate with tissue properties, offering insights into tissue composition.
Purpose of the Study:
- To investigate if tissue modifications alter acoustic properties.
- To evaluate SAM's utility in monitoring chemical changes in tissue sections.
Main Methods:
- Tissue samples underwent formalin fixation, periodic acid-Schiff (PAS) reactions, and pepsin digestion.
- Changes in SOS were measured using SAM and compared with optical histology.
Main Results:
- Formalin fixation and PAS reactions (glycosylation) increased SOS.
- Pepsin digestion decreased SOS, with sensitivity varying based on inflammation and aging.
- SAM detected chemical changes with higher sensitivity than optical histology.
Conclusions:
- SAM can visualize the distribution and time course of chemical modifications in tissues.
- SAM is a sensitive tool for studying tissue alterations like protein cross-linkage, repair, and aging.
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