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The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against

Jana Seele1, Lena-Maria Hillermann1, Andreas Beineke2

  • 1Institute for Microbiology, Department of Infectious Diseases, University of Veterinary Medicine Hannover, Hannover, Germany.

Vaccine
|April 1, 2015
PubMed

Insights

A novel recombinant vaccine using Streptococcus suis's IgM degrading enzyme (IdeSsuis) protected piglets from disease. This IdeSsuis vaccine shows high efficacy against S. suis infections in pigs.

Area of Science:

  • Veterinary Microbiology
  • Immunology
  • Vaccine Development

Background:

  • Streptococcus suis is a significant swine pathogen causing severe diseases like meningitis and arthritis.
  • A specific immunoglobulin M (IgM) degrading enzyme, IdeSsuis, has been identified in S. suis strains.
  • The potential of IdeSsuis as a vaccine candidate against S. suis infections requires investigation.

Purpose of the Study:

  • To evaluate the immunogenicity and protective efficacy of a recombinant IdeSsuis (rIdeSsuis) vaccine in pigs.
  • To assess the impact of rIdeSsuis vaccination on S. suis survival in porcine blood.

Main Methods:

  • Production and administration of recombinant IdeSsuis (rIdeSsuis) vaccine to piglets.
  • Challenge with virulent S. suis serotype 2 and monitoring of clinical outcomes (mortality, morbidity).
  • Measurement of anti-IdeSsuis antibodies and assessment of S. suis killing in porcine blood ex vivo.

Main Results:

  • Vaccination with rIdeSsuis successfully elicited neutralizing antibodies against IdeSsuis.
  • Piglets vaccinated with rIdeSsuis demonstrated complete protection against mortality and severe morbidity following S. suis challenge.
  • rIdeSsuis vaccination significantly enhanced the killing of S. suis in porcine blood, reducing bacterial survival.

Conclusions:

  • Recombinant IdeSsuis (rIdeSsuis) is a highly immunogenic and protective antigen in pigs.
  • The rIdeSsuis vaccine offers a promising strategy for controlling Streptococcus suis infections in swine populations.
Keywords:
Bactericidal assayIgM proteaseNeutralizing antibodiesPigsStreptococcus suis

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