Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of a Stretching Exercise Program on Work-Related Musculoskeletal Disorders in Operating Room Nurses: A Quasi-Experimental Study.

Workplace health & safety·2026
Same author

Endobiota-Estrobolome Profiles in Reproductive-Aged Women With Ovarian Endometriosis.

Reproductive medicine and biology·2026
Same author

Effects of Independent Dynamic Range Compression of Speech and Music Signals on Sound Quality.

American journal of audiology·2026
Same author

Real-world effectiveness of MiSight 1 Day contact lenses in Taiwanese children: a multicenter retrospective study.

Frontiers in medicine·2026
Same author

A Microfluidic Platform for Viscosity Testing of Non-Newtonian Fluids in Engineering and Biomedical Applications.

Micromachines·2026
Same author

Dysregulation of PCSK9 via m<sup>6</sup>A-dependent epitranscriptomic programs fosters hepatocellular carcinoma progression.

Cancer letters·2026

Related Experiment Video

Updated: Apr 15, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.8K

Embryo formation from low sperm concentration by using dielectrophoretic force.

Hong-Yuan Huang, Yu-Hsuan Huang1, Wei-Lun Kao2

  • 1Institute of NanoEngineering and MicroSystems, National Tsing Hua University , Hsinchu 30013, Taiwan.

Biomicrofluidics
|April 1, 2015
PubMed
Summary

This study introduces a novel biochip system using dielectrophoretic forces to enhance in vitro fertilization in mice. The microfluidic device successfully increased fertilization rates, paving the way for improved assisted reproductive technologies.

More Related Videos

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
06:33

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis

Published on: June 5, 2018

7.8K
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
11:15

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Published on: February 16, 2012

12.3K

Related Experiment Videos

Last Updated: Apr 15, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.8K
Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis
06:33

Probing the Roles of Physical Forces in Early Chick Embryonic Morphogenesis

Published on: June 5, 2018

7.8K
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
11:15

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

Published on: February 16, 2012

12.3K

Area of Science:

  • Bioengineering
  • Reproductive Biology
  • Microfluidics

Background:

  • In vitro fertilization (IVF) techniques are crucial for assisted reproduction.
  • Current IVF methods can be limited by sperm-egg interaction efficiency.
  • Mammalian oviductal environment plays a key role in natural fertilization.

Purpose of the Study:

  • To develop a microfluidic biochip system that mimics the mammalian oviduct for in vitro fertilization.
  • To utilize dielectrophoretic (DEP) forces for precise manipulation and positioning of sperm and oocytes.
  • To enhance the probability of natural fertilization by increasing sperm concentration around the oocyte.

Main Methods:

  • Fabrication of microfluidic devices using poly(dimethylsiloxane).
  • Application of positive dielectrophoretic (DEP) force (AC 10 Vpp, 1 MHz, 10 min) to manipulate oocytes and sperm.
  • Numerical simulation using CFDRC-ACE+ to analyze electric field distribution and trapping locations.
  • In vitro fertilization experiments with imprinting-control-region (ICR) mice sperm and oocytes.

Main Results:

  • Successful simultaneous manipulation and positioning of oocytes and sperm using positive DEP force.
  • Achieved an average fertilization rate of 51.58% in the microchannel system.
  • Demonstrated embryo development to the two-cell stage within 24 hours and four-cell stage within 48 hours.
  • Optimized insemination concentration at 1.5 × 10^6 sperm/mL.

Conclusions:

  • The developed microfluidic biochip system effectively simulates oviductal conditions for in vitro fertilization.
  • Positive dielectrophoresis is a viable method for enhancing sperm-egg interaction and fertilization rates.
  • This technology holds promise for improving the efficiency of assisted reproductive technologies.