Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

11.1K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
11.1K
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

32.6K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
32.6K
Free-Radical Chain Reaction and Polymerization of Alkenes02:35

Free-Radical Chain Reaction and Polymerization of Alkenes

9.4K
The conversion of alkenes to macromolecules called polymers is a reaction of high commercial importance. The structure of the polymer is defined by a repeating unit, while the terminal groups are considered insignificant. The average degree of polymerization represents the number of repeating units in the polymer molecule and is denoted by the subscript n.
9.4K
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

14.7K
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
14.7K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

10.8K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A real-world feasibility study: at home longitudinal use of the Cumulus NeuLogiq® platform for electrophysiological and neurocognitive measures in patients with mild Alzheimer's Disease dementia.

Frontiers in digital health·2026
Same author

Longitudinal cognitive assessment using the Cumulus NeuLogiq platform in amyotrophic lateral sclerosis and frontotemporal dementia.

Scientific reports·2026
Same author

Results of a Phase 1 Study Assessing the Effect of CIN-102, a Novel Formulation of the Dopamine Receptor Antagonist Domperidone Designed to Treat Gastroparesis, on Cardiac Repolarization in Healthy Volunteers.

Clinical pharmacology in drug development·2026
Same author

Treatment of non-effusive feline infectious peritonitis using oral remdesivir or GS-441524: a randomized, double-blind, non-inferiority trial.

Journal of feline medicine and surgery·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Beyond macrophages: FIPV tropism includes T and B lymphocytes.

Veterinary microbiology·2026

Related Experiment Video

Updated: Jan 23, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
08:46

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Published on: August 26, 2020

5.3K

Automated Microfluidic Platform for Serial Polymerase Chain Reaction and High-Resolution Melting Analysis.

Weidong Cao1, Brian Bean2, Scott Corey3

  • 1Canon U.S. Life Sciences, Inc, Rockville, MD, USA.

Journal of Laboratory Automation
|April 2, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces an automated genetic analyzer for human sample testing. It combines microfluidic rapid polymerase chain reaction (PCR) and high-resolution melting analysis (HRMA) for faster, high-quality results.

Keywords:
PCRhigh-resolution melting analysisliquid handlingmicrofluidic

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.5K

Related Experiment Videos

Last Updated: Jan 23, 2026

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
08:46

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

Published on: August 26, 2020

5.3K
Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay
08:22

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

10.2K
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

11.5K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Genetics

Background:

  • Automated genetic analysis is crucial for human sample testing.
  • Current methods for polymerase chain reaction (PCR) and high-resolution melting analysis (HRMA) can be time-consuming.
  • Microfluidic platforms offer potential for faster and more efficient molecular analysis.

Purpose of the Study:

  • To develop an automated genetic analyzer integrating microfluidic PCR and HRMA.
  • To enhance the speed and efficiency of human genetic sample testing.
  • To achieve high-quality data suitable for clinical applications.

Main Methods:

  • Development of an integrated DNA microfluidic cartridge and a robotic pipettor system.
  • Implementation of a novel image feedback flow control system using a digital camera for valve-less fluid control.
  • Utilizing the same camera for high-resolution melt curve analysis of DNA amplicons.
  • Serial PCR and HRMA performed on a microfluidic platform.

Main Results:

  • Dramatically reduced time frame for PCR and HRMA from hours to minutes.
  • Achieved high data quality suitable for human sample testing.
  • Demonstrated feasibility of rapid serial PCR and HRMA.

Conclusions:

  • The developed automated genetic analyzer significantly accelerates human sample testing.
  • The integrated microfluidic system with image feedback control enables rapid and high-quality genetic analysis.
  • This technology holds promise for efficient and reliable genetic diagnostics.