Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

19.0K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
19.0K
Redox Reactions01:27

Redox Reactions

1.4K
Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...
1.4K
Oxidation and Reduction of Organic Molecules01:19

Oxidation and Reduction of Organic Molecules

10.8K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
10.8K
Electron Carriers01:24

Electron Carriers

95.3K
Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
95.3K
The Electron Transport Chain01:30

The Electron Transport Chain

21.8K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
21.8K
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

9.9K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bisindolylmaleimide Ligands Stabilize <i>c-MYC</i> G-Quadruplex DNA Structure and Downregulate Gene Expression.

Biochemistry·2022
Same author

The emerging role of nebulization for maintenance treatment of chronic obstructive pulmonary disease at home.

Lung India : official organ of Indian Chest Society·2021
Same author

Immediate effect of horse riding simulator on adductor spasticity in children with cerebral palsy: A randomized controlled trial.

Physiotherapy research international : the journal for researchers and clinicians in physical therapy·2019
Same author

Synthesis of the Dynamical Properties of Feedback Loops in Bio-Pathways.

IEEE/ACM transactions on computational biology and bioinformatics·2019
Same author

Knowledge gaps persist and hinder progress in eliminating mumps.

Vaccine·2018
Same author

Improving the lean muscle color of dark-cutting beef by aging, antioxidant-enhancement, and modified atmospheric packaging.

Journal of animal science·2018

Related Experiment Video

Updated: Apr 15, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
08:03

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Published on: March 7, 2025

1.1K

Reverse electron transport effects on NADH formation and metmyoglobin reduction.

K M Belskie1, C B Van Buiten1, R Ramanathan2

  • 1Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.

Meat Science
|April 2, 2015
PubMed
Summary

Succinate and NAD generate NADH in bovine heart mitochondria through reverse electron flow. This NADH supports both electron transport-mediated and metmyoglobin reductase pathways postmortem.

Keywords:
Meat colorMetmyoglobin reductionMitochondriaMyoglobinOxygen consumption

More Related Videos

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
10:27

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

7.4K
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.7K

Related Experiment Videos

Last Updated: Apr 15, 2026

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
08:03

Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays

Published on: March 7, 2025

1.1K
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
10:27

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Published on: May 4, 2018

7.4K
Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

19.7K

Area of Science:

  • Biochemistry
  • Mitochondrial Function

Background:

  • Metmyoglobin reductase activity is crucial for reducing methemoglobin.
  • Mitochondria play a key role in cellular energy metabolism and redox balance.

Purpose of the Study:

  • To investigate if NADH generated via mitochondrial reverse electron flow can fuel metmyoglobin reduction pathways.
  • To determine the role of NADH in postmortem bovine heart tissue metabolism.

Main Methods:

  • Isolation of beef heart mitochondria.
  • Measurement of oxygen consumption and NADH formation using succinate, NAD, and inhibitors (antimycin A, rotenone).
  • Assay of electron transport-mediated metmyoglobin reduction and reductase activity.

Main Results:

  • Succinate and NAD significantly increased oxygen consumption, NADH formation, and metmyoglobin reduction.
  • Antimycin A inhibited electron flow, decreasing oxygen consumption and metmyoglobin reduction.
  • Rotenone blocked reverse electron flow, increasing oxygen consumption and metmyoglobin reduction while decreasing NADH formation.

Conclusions:

  • NADH generated by reverse electron flow in bovine mitochondria can be utilized by both electron transport-mediated and metmyoglobin reductase pathways.
  • This finding has implications for understanding postmortem biochemical processes in meat tissues.