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Novel strategy for herbal species classification based on UPLC-HRMS oligosaccharide profiling
Cai Tie1, Ting Hu1, Baolin Guo2
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing 100050, PR China.
Journal of Pharmaceutical and Biomedical Analysis
|April 2, 2015
Summary
A new method enhances oligosaccharide analysis in Epimedium herbs using derivatization and UPLC-HRMS. This technique improves detection sensitivity and aids in classifying Epimedium species by their unique oligosaccharide profiles.
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Pharmacology
Background:
- Oligosaccharides possess significant pharmacological activities but are challenging to analyze due to their complexity.
- Accurate profiling and quality control of oligosaccharides are crucial for understanding their bioactivity.
Purpose of the Study:
- To develop a novel, rapid analytical method for profiling and identifying oligosaccharides in Epimedium species.
- To enhance the sensitivity and efficiency of oligosaccharide analysis using derivatization coupled with UPLC-HRMS.
Main Methods:
- A new derivatization pretreatment using 2,4-bis(diethylamino)-6-hydrazino-1,3,5-triazine for oligosaccharides.
- Ultra-performance liquid chromatography coupled with high-resolution tandem mass spectrometry (UPLC-HRMS/MS) for analysis.
- Principal Component Analysis (PCA) for statistical processing of oligosaccharide data from 52 Epimedium samples.
Main Results:
- The derivatization significantly increased MS response (two orders of magnitude) for oligosaccharides.
- Successfully profiled and identified 66 oligosaccharide compounds across 52 Epimedium samples.
- Oligosaccharide profiles allowed for the classification of different Epimedium species.
Conclusions:
- The developed UPLC-HRMS method with derivatization is effective for rapid oligosaccharide profiling and identification in Epimedium.
- Thirteen specific oligosaccharide compounds show potential as biomarkers for Epimedium species classification.
- This approach facilitates quality control and species differentiation of medicinal herbs.
