Highly Emissive Nanoparticles Based on AIE-Active Molecule and PAMAM Dendritic "Molecular Glue"
Xinyang Liu1, Yi Zeng1, Jun Liu1
1†Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, and ‡Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
Abstract:
The highly emissive nanoparticles Gn-TCMPE (n = 0-4) were prepared by using PAMAM dendrimers as "molecular glue" to adhere an AIE-active molecule tetra(4-(carboxymethoxy)phenyl)ethylene (TCMPE). The electrostatic interaction of ammonium-carboxylate ion pairs provides a driving force between TCMPE and PAMAM dendrimers to form the nanoparticles Gn-TCMPE (n = 0-4), which is validated by the FTIR and (1)H NMR spectra. The formation of nanoparticles dramatically blocks the nonradiative pathway and enhances the fluorescence of TCMPE. The quantum yields of Gn-TCMPE gradually boost at first and then reach to a plateau with increasing the generation of PAMAM dendrimers, and the highest absolute quantum yields are obtained to be 0.42 and 0.64 for Gn-TCMPE (n = 2-4) in methanol dispersion and solid phases, respectively. The fluorescence of the nanoparticles can be tuned by addition of trifluoroacetic acid (TFA). Furthermore, the G4-TCMPE has been successfully applied to selectively image cytoplasm of Hela cells with excellent photostability and low cytotoxicity. This study provides a novel noncovalent strategy for developing highly emissive and robust organic materials fitting for cell fluorescence imaging.
