Related Experiment Video
Updated: Apr 15, 2026

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping
Published on: December 8, 2015
Dark field transmission electron microscopy as a tool for identifying inorganic nanoparticles in biological matrices
Nathan D Klein1, Katie R Hurley1, Z Vivian Feng2
1†University of Minnesota, Department of Chemistry, 207 Pleasant St. SE, Minneapolis, Minnesota 55455, United States.
Abstract:
Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.
More Related Videos
14:53In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
Published on: February 3, 2018
10:17Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue
Published on: June 18, 2014
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Transmission Electron Microscopy
Overview of Electron Microscopy
Immunogold Electron Microscopy