Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

3.8K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
3.8K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.9K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.9K
Integrins01:10

Integrins

6.2K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
6.2K
Activation of Integrins01:15

Activation of Integrins

5.7K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
5.7K
Anchoring Junctions01:03

Anchoring Junctions

5.7K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
5.7K
Adherens Junctions01:24

Adherens Junctions

7.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Glycemic index, glycemic load, and colorectal cancer risk stratified by insulin receptor expression: the Japan Public Health Center-based Prospective study.

European journal of nutrition·2026
Same author

Gas temperature measurement based on contrast reversal in mid-infrared CO<sub>2</sub> images.

Optics express·2026
Same author

Effect of Fermented Papaya Preparation Intake on Fatigue Recovery, Useful Field of View and Oxidative Stress: A Pilot Interventional Study in Professional Bus and Motor Racing Drivers.

Journal of the American Nutrition Association·2026
Same author

Corrigendum to 'Identification of cell type specific marker genes of dental epithelial cells using single cell RNA sequence: a Review' [J. Oral Biosci. 68, (2026) 100757].

Journal of oral biosciences·2026
Same author

Case Report: Exploring the link between severe gingivitis and inflammatory bowel disease in a young patient.

Frontiers in dental medicine·2026
Same author

Fibroblast growth factor 8 regulates early Meckel's cartilage development via ERK signaling in a stage- and dose-dependent manner.

Developmental biology·2026

Related Experiment Video

Updated: Apr 15, 2026

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
08:01

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Published on: August 14, 2015

8.7K

Interaction between fibronectin and β1 integrin is essential for tooth development.

Kan Saito1, Emiko Fukumoto1, Aya Yamada1

  • 1Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry, Sendai, Japan.

Plos One
|April 2, 2015
PubMed
Summary

Fibronectin and β1 integrin interactions are crucial for dental epithelial cell binding and tooth formation. Conditional knockout of β1 integrin in mice led to enamel hypoplasia and delayed tooth eruption, highlighting fibronectin

More Related Videos

Isolation of Epithelial Cells from Human Dental Follicle
04:07

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

3.4K
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
07:47

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Published on: November 13, 2013

14.8K

Related Experiment Videos

Last Updated: Apr 15, 2026

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices
08:01

Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture Devices

Published on: August 14, 2015

8.7K
Isolation of Epithelial Cells from Human Dental Follicle
04:07

Isolation of Epithelial Cells from Human Dental Follicle

Published on: November 5, 2021

3.4K
The Slice Culture Method for Following Development of Tooth Germs In Explant Culture
07:47

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Published on: November 13, 2013

14.8K

Area of Science:

  • Developmental Biology
  • Oral Biology
  • Extracellular Matrix Research

Background:

  • Dental epithelium and extracellular matrix interactions are vital for tooth development.
  • Fibronectin's role in dental epithelial differentiation and tooth formation requires elucidation.

Purpose of the Study:

  • To investigate the function of fibronectin in dental epithelial differentiation and tooth formation.
  • To determine the specific role of fibronectin-β1 integrin interactions in vivo.

Main Methods:

  • Analysis of fibronectin mRNA expression in developing incisors.
  • In vitro binding assays using dental epithelial cells and fibronectin-coated dishes.
  • Generation and analysis of β1 integrin conditional knockout mice (Intβ1lox-/lox-/K14-Cre).

Main Results:

  • Fibronectin expression varied during different stages of tooth development.
  • Fibronectin-β1 integrin interactions mediated dental epithelial cell binding.
  • β1 integrin knockout mice showed enamel hypoplasia, delayed eruption, and impaired ameloblast differentiation.
  • Knockout cells exhibited reduced fibronectin binding and inhibited ameloblastin expression.

Conclusions:

  • Fibronectin-β1 integrin interactions are essential for proper dental epithelial cell binding and differentiation.
  • These interactions play a critical role in ameloblast differentiation and enamel formation during tooth development.
  • Targeting fibronectin-β1 integrin pathways may offer therapeutic strategies for dental developmental defects.