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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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GNeosomes: Highly Lysosomotropic Nanoassemblies for Lysosomal Delivery
Ezequiel Wexselblatt1, Jeffrey D Esko1, Yitzhak Tor1
1†Chemistry and Biochemistry and ‡Cellular and Molecular Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
ACS Nano
|April 2, 2015
Summary
Guanidinoneomycin-coated vesicles (GNeosomes) efficiently deliver cargo to lysosomes, utilizing cell surface sugars for uptake. This technology offers a versatile platform for research and therapeutics.
Area of Science:
- Biotechnology
- Nanomedicine
- Cell Biology
Background:
- Lysosomal delivery is crucial for treating various diseases.
- Current methods for lysosomal cargo delivery face limitations.
- Guanidinoneomycin-based lipid vesicles (GNeosomes) offer a novel approach.
Purpose of the Study:
- To characterize GNeosomes as a platform for cellular internalization and lysosomal delivery.
- To investigate the mechanism of GNeosome uptake and cargo release.
- To evaluate the potential of GNeosomes as research tools and therapeutic vehicles.
Main Methods:
- GNeosomes were engineered with guanidinoneomycin for cargo encapsulation.
- Cellular uptake, lysosomal trafficking, and cargo activity were quantified.
- The role of cell surface glycosaminoglycans in GNeosome internalization was assessed.
Main Results:
- GNeosomes efficiently delivered diverse cargo, from small molecules to proteins, into cells.
- Uptake was exclusively dependent on cell surface glycosaminoglycans.
- Co-localization with lysosomes and cargo activity were confirmed.
Conclusions:
- GNeosomes represent a universal platform for efficient lysosomal delivery.
- This technology has significant potential as a basic research tool.
- GNeosomes show promise as a therapeutic vehicle for lysosomal targeting.
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