Related Experiment Video

Updated: Apr 15, 2026

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.6K

Decreased expression of RPS15A suppresses proliferation of lung cancer cells

Xinmin Zhao1,2, Lei Shen2,3, Yu Feng1,2

  • 1Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|April 3, 2015
PubMed

Insights

Ribosomal protein S15a (RPS15A) plays a key role in lung cancer development. Downregulating RPS15A inhibits lung adenocarcinoma cell proliferation and survival, suggesting its potential as a gene therapy target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Expression

Background:

  • Lung cancer is a leading cause of cancer-related mortality worldwide.
  • Ribosomal protein S15a (RPS15A) was previously identified as a TGF-β-responsive gene in A549 lung adenocarcinoma cells.

Purpose of the Study:

  • To investigate the role of RPS15A in lung adenocarcinoma proliferation and survival.
  • To evaluate RPS15A as a potential therapeutic target for lung cancer.

Main Methods:

  • Specific small interfering RNA (si-RNA) was used to downregulate RPS15A expression in A549 cells.
  • Cell proliferation and survival were assessed using MTT and colony formation assays.
  • Cell cycle analysis was performed to determine the effect of RPS15A knockdown on cell cycle progression.
  • Immunohistochemistry was used to examine RPS15A expression in human lung cancer tissues.

Main Results:

  • Decreased RPS15A expression significantly inhibited A549 cell proliferation and survival.
  • RPS15A knockdown led to cell cycle arrest at the G0/G1 phase.
  • RPS15A was found to be overexpressed in surgically resected lung cancer tissues.

Conclusions:

  • RPS15A is identified as a novel oncogenic gene involved in lung carcinogenesis.
  • RPS15A inhibition demonstrates potential for lung cancer gene therapy.
Keywords:
Lung cancerProliferationRPS15Asi-RNA

More Related Videos

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

960
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.6K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
5.0K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.7K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

6.1K