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Related Experiment Video

Updated: Apr 15, 2026

Vinyl Chloride and High-Fat Diet as a Model of Environment and Obesity Interaction
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The carbon tetrachloride model in mice.

D Scholten1, J Trebicka2, C Liedtke1

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Summary

Standardized operation procedures for liver fibrosis research are crucial for animal welfare. This study presents SOPs for common models like carbon tetrachloride (CCl4) to improve data comparability and reduce animal use.

Keywords:
cirrhosiscytochrome P450fibrosishepatotoxinliver damage

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Area of Science:

  • Hepatology
  • Toxicology
  • Animal Models

Background:

  • European animal welfare regulations necessitate updated experimental procedures.
  • Liver fibrosis research requires standardized protocols for reliable results.
  • The Collaborative Research Centre 'Organ Fibrosis: From Mechanisms of Injury to Modulation of Disease' (SFB/TRR57) has extensive experience.

Purpose of the Study:

  • To present standard operation procedures (SOPs) for established liver fibrosis models.
  • To enhance the standardization and comparability of experimental liver fibrosis research.
  • To support the reduction, refinement, and replacement (3R) principles in animal experimentation.

Main Methods:

  • Development of SOPs based on long-term expertise.
  • Focus on widely accepted experimental models for liver injury induction.
  • Detailed description of the carbon tetrachloride (CCl4) model in mice.

Main Results:

  • A series of SOPs for liver fibrosis induction models are provided.
  • The carbon tetrachloride (CCl4) model in mice is detailed as a primary example.
  • The SOPs aim to standardize experimental approaches.

Conclusions:

  • Standardized SOPs are essential for reproducible liver fibrosis research.
  • These SOPs facilitate data comparability across laboratories.
  • Implementation of SOPs aids in reducing animal experimentation while adhering to the 3R principle.