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Related Concept Videos

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Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
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On the complex point spread function in interferometric cross-polarisation microscopy.

Benjamin T Miles, Xin Hong, Henkjan Gersen

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    |April 4, 2015
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    Summary
    This summary is machine-generated.

    This study measures the amplitude and phase response of an Interferometric Cross-polarisation Microscope. The technique provides high-resolution, confocal-like images of living cells, crucial for advanced microscopy applications.

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    Area of Science:

    • Microscopy
    • Biophysics
    • Optical Physics

    Background:

    • Characterizing the Point Spread Function (PSF) is essential in microscopy.
    • Detecting fields, not just intensities, requires understanding complex PSFs.

    Purpose of the Study:

    • To experimentally measure and theoretically model the volumetric amplitude and phase response of an Interferometric Cross-polarisation Microscope.
    • To demonstrate its capability in imaging weakly birefringent structures in living cells.

    Main Methods:

    • Interferometric Cross-polarisation Microscopy was employed.
    • Experimental measurements and theoretical modeling of amplitude and phase response were performed.

    Main Results:

    • The axial Full Width at Half Maximum (FWHM) of the amplitude PSF was measured to be 0.83 μm and modeled as 0.70 μm.
    • The microscope achieved resolution comparable to confocal microscopy.

    Conclusions:

    • Interferometric Cross-polarisation Microscopy can provide confocal-like images of living cells.
    • Retaining both amplitude and phase information can enhance object localization in microscopy.