Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

1.3K
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
1.3K
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

5.7K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
5.7K
Calibration Curves: Correlation Coefficient01:10

Calibration Curves: Correlation Coefficient

6.5K
In a linear calibration curve, there is a value called the calibration coefficient, denoted by 'r,' which measures the strength and the direction of association between two variables. The correlation coefficient value ranges from −1 to +1. A value of +1 indicates a perfect positive linear correlation, −1 denotes a perfect negative correlation, and 0 implies no correlation between the two variables. A positive correlation value establishes that as one variable increases, the...
6.5K
Glassware Calibration01:11

Glassware Calibration

2.1K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
2.1K
Electronic Distance Measuring Instruments01:30

Electronic Distance Measuring Instruments

702
Electronic Distance Measuring Instruments (EDMs) are essential tools in modern surveying, offering precise distance measurements by emitting electromagnetic signals and calculating the time required for these signals to travel to a target and return. Two primary types of signals are used in EDMs — light waves and microwaves — each suited to specific environmental and distance requirements. Light-wave-based EDMs utilize either infrared or laser light, providing high accuracy over...
702
Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

55.9K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
55.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laser speckle imaging for biofilm viability assessment: a noninvasive alternative to the MTT assay.

Biomedical optics express·2026
Same author

Accurate and noise-robust wavefront reconstruction with an optical vortex wavefront sensor.

Optics express·2026
Same author

Defocused speckle imaging using digital image correlation for lensless tip and tilt measurements with experimental validation.

Applied optics·2025
Same author

Improved temporal speckle contrast model for slow and fast dynamic: effect of temporal correlation among neighboring pixels.

Journal of biomedical optics·2025
Same author

Relationship between bone quality and shrinkage in maxillary sinus augmentation using synthetic alloplast versus xenograft.

Journal of periodontal & implant science·2025
Same author

Single-shot quantitative phase microscopy: a multi-functional tool for cell analysis.

Biomedical optics express·2024

Related Experiment Video

Updated: Apr 15, 2026

Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

Published on: April 26, 2014

9.9K

Self-calibrating common-path interferometry.

Rosario Porras-Aguilar, Konstantinos Falaggis, Julio C Ramirez-San-Juan

    Optics Express
    |April 4, 2015
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new quantitative phase imaging technique using random phase shifts in common-path interferometers. The method leverages nonlinear liquid crystals for cost-effective, precise phase measurements without calibration.

    More Related Videos

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.7K

    Related Experiment Videos

    Last Updated: Apr 15, 2026

    Implementation of a Reference Interferometer for Nanodetection
    16:11

    Implementation of a Reference Interferometer for Nanodetection

    Published on: April 26, 2014

    9.9K
    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
    11:57

    Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

    Published on: December 1, 2016

    11.3K
    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
    12:14

    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    22.7K

    Area of Science:

    • Optical physics
    • Phase imaging
    • Interferometry

    Background:

    • Quantitative phase imaging is crucial for analyzing transparent specimens.
    • Common-path interferometers offer stability but often require precise phase shift calibration.
    • Existing methods may not fully account for complex image formation in non-ideal setups.

    Purpose of the Study:

    • To develop a quantitative phase measuring technique for common-path interferometers.
    • To enable phase imaging without the need for explicit phase shift calibrations.
    • To utilize nonlinear liquid crystal materials for phase filtering in interferometry.

    Main Methods:

    • A novel random phase shifting algorithm based on Generalized Phase Contrast theory was derived.
    • Phase-shifted interferograms were acquired using a common-path configuration.
    • Nonlinear liquid crystal materials were employed as self-induced phase filters.

    Main Results:

    • The technique successfully estimates object phase from interferograms with unknown phase shifts.
    • Experimental validation demonstrated the technique's efficacy in common-path setups.
    • The use of liquid crystal phase filters eliminated the need for phase shift calibrations.

    Conclusions:

    • The presented quantitative phase measuring technique is robust for common-path interferometers.
    • Nonlinear liquid crystal phase filters offer advantages like cost-effectiveness and self-alignment.
    • This method advances quantitative phase imaging by simplifying calibration requirements.