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Updated: Apr 15, 2026
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Published on: August 9, 2017
Detection of mouse natural killer T follicular helper (NKT(FH)) cells by flow cytometry
Pheh-Ping Chang1, Carola G Vinuesa
1Department of Pathogens and Immunity, John Curtin School of Medical Research, Australian National University, Building 131, Garran Road, ACTON, GPO Box 334, Canberra, Australia, pheh.chang@anu.edu.au.
Abstract:
Natural killer T follicular helper (NKT(FH)) cell, a recently identified B-cell helper innate cell population, can be easily missed due to its low frequency and the fact that it only forms upon immunization or infection with glycolipid-containing antigens or microbes. Here, we describe our in-house optimized protocol to detect these mouse NKT(FH) cells by multiparameter flow cytometer.
