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Updated: Apr 15, 2026

Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper cTfh Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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Published on: January 7, 2019

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Quantifying helper cell function of human TFH cells in vitro

Nina Chevalier1

  • 1Department of Rheumatology and Clinical Immunology, University Freiburg Medical School, Hugstetter Str. 55, Freiburg, 79106, Germany, nina.chevalier@uniklinik-freiburg.de.

Methods in Molecular Biology (Clifton, N.J.)
|April 4, 2015
PubMed

Blood-circulating CXCR5(+) CD4(+) T cells and T follicular helper (TFH) cells, which participate in germinal center (GC) reactions within secondary lymphoid organs, are specialized in providing help to B cells. This chapter describes ways to isolate TFH-like cells out of peripheral blood or tonsils and to quantify their B cell helper function. This comprises different co-culture approaches of TFH-like cells and B cells and the evaluation of their capacity to induce immunoglobulin secretion and plasma cell differentiation. In addition, B cell helper function of CD4 T cells can be estimated indirectly by quantifying the expression of B cell helper cytokines and co-stimulatory and TFH-associated molecules.

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