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Transcriptome analysis and gene expression profiling of abortive and developing ovules during fruit development in
Yunqing Cheng1, Jianfeng Liu1, Huidi Zhang1
1College of Life Sciences, Jilin Normal University, Siping, Jilin Province 136000, China.
Plos One
|April 4, 2015
Summary
Hazelnut blank fruit, a common issue causing yield loss, is linked to embryo abortion. This study used transcriptome sequencing to identify key genes involved in hazelnut ovule development and abortion, offering insights into this phenomenon.
Area of Science:
- Plant Science
- Genomics
- Molecular Biology
Background:
- High incidence of blank fruit in hazelnut (Corylus heterophylla Fisch) leads to significant yield reduction in northeast China.
- Embryo abortion is a known cause of blank fruit development in the Corylus genus, but molecular mechanisms remain unclear.
- Lack of genomic information for C. heterophylla Fisch necessitates transcriptome and gene expression profiling.
Purpose of the Study:
- To investigate the molecular mechanisms underlying embryo abortion in developing hazelnut ovules.
- To generate transcriptome and gene expression data for normally developing and abortive hazelnut ovules.
- To identify differentially expressed genes associated with ovule abortion.
Main Methods:
- De novo transcriptome sequencing using Illumina HiSeq 2000.
- RNA-seq analysis to assemble unigenes and identify genetic information related to fruit development.
- Construction of digital gene expression libraries for developing and abortive ovules.
- Annotation of unigenes using public databases (NR, NT, Swiss-Prot, KEGG, COG, GO).
- Quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression validation.
Main Results:
- Assembly of 55,353 unigenes from transcriptome sequencing.
- Identification of 1,637 upregulated and 715 downregulated unigenes in abortive ovules compared to developing ovules.
- Confirmation of differential gene expression through qRT-PCR.
Conclusions:
- The study provides comprehensive transcriptome and digital gene expression data for hazelnut ovules.
- This data enhances understanding of the molecular basis of abortive ovule formation in hazelnut.
- Findings contribute to addressing yield losses caused by blank fruit in hazelnut production.
