Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early targets and progressive deterioration in cardiac performance in response to chronically modified cardiac troponin I.

American journal of physiology. Heart and circulatory physiology·2026
Same author

A learning health system approach to improving chronic kidney disease management in primary care.

BMC primary care·2026
Same author

Bridging the Evidence and Practice Gap in Chronic Kidney Disease: A System Thinking Approach to Population Health.

Population health management·2024
Same author

Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes.

Journal of visualized experiments : JoVE·2022
Same author

Cardiac contractile dysfunction and protein kinase C-mediated myofilament phosphorylation in disease and aging.

The Journal of general physiology·2019
Same author

Troponin I modulation of cardiac performance: Plasticity in the survival switch.

Archives of biochemistry and biophysics·2019

Related Experiment Video

Updated: Apr 15, 2026

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening
05:06

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening

Published on: May 24, 2024

1.3K

Gene transfer into cardiac myocytes.

Sarah E Lang1, Margaret V Westfall

  • 1Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI, 481091, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 4, 2015
PubMed
Summary

Gene transfer using recombinant adenovirus offers an efficient method for introducing genetic material into cardiac myocytes, overcoming limitations of traditional DNA transfection techniques for these specialized cells.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Cellular Biology

Background:

  • Traditional DNA transfection methods exhibit low efficiency and toxicity in terminally differentiated cells, particularly cardiac myocytes.
  • Cardiac myocytes present a significant challenge for genetic manipulation due to their specialized, differentiated state.

Purpose of the Study:

  • To provide detailed protocols for the isolation of adult rat cardiac myocytes.
  • To establish an efficient vector-based gene transfer method for cardiac myocytes.
  • To facilitate the study of sarcomeric proteins and their impact on myofilament function.

Main Methods:

  • Isolation of adult rat cardiac myocytes.
  • Gene transfer utilizing recombinant adenovirus vectors.
  • Analysis of myofilament function.

More Related Videos

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
12:15

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

Published on: September 17, 2009

15.1K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

9.2K

Related Experiment Videos

Last Updated: Apr 15, 2026

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening
05:06

Author Spotlight: Cardiac Cell Transgenesis for Rapid Gene Screening

Published on: May 24, 2024

1.3K
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging
12:15

Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging

Published on: September 17, 2009

15.1K
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
10:05

Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5

Published on: November 13, 2015

9.2K

Main Results:

  • The described protocols enable efficient gene transfer into primary cardiac myocyte cultures.
  • Recombinant adenovirus facilitates the introduction of exogenous cDNA into cardiac myocytes.
  • The method is suitable for investigating the functional effects of sarcomeric proteins.

Conclusions:

  • Vector-based gene transfer with recombinant adenovirus is a viable and efficient strategy for genetic manipulation of cardiac myocytes.
  • This approach overcomes the limitations of traditional transfection methods for studying cardiac myocyte function.